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> MPO, Human, ELISA kit

MPO, Human, ELISA kit

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Référence HK324-01

Conditionnement : 1x96det.

Marque : Hycult Biotech

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Téléphone : +1 850 650 7790

MPO, Human, ELISA kit-0
MPO, Human, ELISA kit-726

MPO, Human, ELISA kit

The human MPO ELISA kit is to be used for the in vitro quantitative determination of human MPO in plasma, sputum and cell culture supernatant samples.

Advanced insights with the MPO Human ELISA kit

The MPO human ELISA offers precise MPO quantification in human samples. It measures MPO, a key glycoprotein, with unparalleled accuracy. MPO is essential in myeloid cells. It is found in neutrophils’ azurophilic granules. MPO is crucial for the body’s defense. It aids in phagocytic lysis and produces HOCl, a strong oxidant. Activated neutrophils release MPO rapidly.

MPO’s role is vast. It is linked to diseases like atherosclerosis and lung cancer. It is also connected to Alzheimer’s and multiple sclerosis. In Wegener’s disease, it is targeted by autoimmune antibodies. MPO deficiency, once overlooked, is now a key research area.

The classical MPO assay is an enzymatic assay for activity of MPO. This classical MPO assay is hampered by the presence of inhibitory compounds in tissue homogenates and plasma. In this type of assays spiking often gives unreliable results. The human MPO ELISA is not influenced by inhibitors of the enzyme activity.

Perform curve fitting with Assayfit Pro with the ELISA calculation sheets.

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Application
The human MPO ELISA kit is to be used for the in vitro quantitative determination of human MPO in plasma, sputum and cell culture supernatant samples.

Principle
The human MPO ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human MPO. Biotinylated tracer antibody will bind to captured human MPO. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human MPO standards (log). The human MPO concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

Product type
Assays
Amount
1 x 96 det., 2 x 96 det.
Standard range
0.4 to 100 ng/ml
Detection level
0.4 ng/ml
Working volume
100 µl/well
Species
Human
Cross reactivity
Baboon – No, Horse – No, Mouse – No, Pig – No, Rabbit – No, Rat – No, Sheep – No
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
Disease
Cardiology and metabolism, Pulmonology
Safety Data Sheet
SDS - HK324
61 kb
Assay Manual
HK324
401 kb

Calculate your ELISA data easily

With the ELISA calculator you can easily calculate ELISA data. Assayfit Pro helps to perform curve fitting. The calculator generates advanced reports, fit graph, fit parameters and goodness of fit are shown.

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