OCOA02393 | NODAL Protein Lysate Clinisciences

Datasheets/Manuals	Printable datasheet for NODAL Protein Lysate (OCOA02393)

Product Info
Predicted Species Reactivity	Homo sapiens
Product Format	Lyophilized
Host	HEK293T
Additional Information	Components: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::	Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and Storage	The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
Tag	C-MYC/DDK

Gene Symbol	NODAL
Gene Full Name	nodal growth differentiation factor
Alias Symbols	HTX5;nodal homolog.
NCBI Gene Id	4838
Protein Name	NM_018055, NP_060525, Q96S42
Description of Target	Homo sapiens nodal growth differentiation factor (NODAL), transcript variant 1, mRNA, Nodal homolog, nodal homolog isoform 1 preproprotein [Homo sapiens]
Uniprot ID	https://www.uniprot.org/uniprot/Q96S42
Protein Accession #	https://www.ncbi.nlm.nih.gov/protein/NP_060525
Nucleotide Accession #	https://www.ncbi.nlm.nih.gov/nuccore/NM_018055
Molecular Weight	39.58 kDa

NODAL Protein Lysate