Parathyroid Hormone 1-34 Antibody (Human) ELISA Kit

Référence OKCA00498

Conditionnement : 96Wells

Marque : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit PrincipleAviva Systems Biology Parathyroid Hormone 1-34 Antibody (Human) ELISA Kit (OKCA00498) is based on standard reverse capture sandwich enzyme-linked immuno-sorbent assay technology. Parathyroid Hormone 1-34 antigen has been pre-coated and blocked in a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed. An HRP conjugated detector antibody specific for HumanAntibody is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Parathyroid Hormone 1-34 Antibody captured in well.
ELISA Kit ReproducibilityThree different samples of known Parathyroid Hormone 1-34 Antibody concentration were tested across one plate with 20 replicates and across 3 plates, 8 replicates each.
Intra-Assay CV: <15%
Inter-Assay CV: <15%
ELISA Kit Component
ComponentAmount
Parathyroid Hormone 1-34 Antibody Microplate96 Wells (12 x 8 Well strips)
Negative Control 1 x 800 ul
Positive Control 1 x 800 ul
100X Anti-Human Antibody HRP-conjugate1 x 120 ul
HRP-conjugate Diluent 1 x 20 ml
Sample Diluent 2 x 20 ml
25X Wash Buffer1 x 20 ml
TMB Substrate 1 x 10 ml
Stop Solution 1 x 10 ml
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum and Plasma
Assay InfoAssay Methodology: Qualitative Reverse Capture ELISA
Protocol Information
  • Reconstitution & Storage Instructions
  • Western Blotting/Immunoblotting (WB/IB) Protocol
  • Immunohistochemistry (IHC) Protocol
  • Immunocytochemistry (ICC) Protocol
  • Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
  • Blocking Peptide Competition Protocol (BPCP)
  • Immunoprecipitation (IP) Protocol
  • Antibody Array (AA) Protocol
Alias SymbolsPTH 1-34
Protein NameParathyroid Hormone 1-34 Antibody
Description of TargetParathyroid Hormone 1-34 Antibody

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