PS100093 | pLenti-C-mGFP-P2A-Puro Lentiviral Gene Expression Vector

pLenti-C-mGFP-P2A-Puro Lentiviral Gene Expression Vector

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SKU

PS100093

pLenti-C-mGFP-P2A-Puro, Lenti vector with C-terminal mGFP tag and P2A-Puro, 10 ug

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Datasheet SDS Vector Sequence

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Specifications

Specifications
Product Data
C-Tag	mGFP
E. coli Selection	Chloramphenicol (34 ug/mL)
Mammalian Cell Selection	Puromycin
Features	This Lenti vector is a third generation lentiviral vector. All OriGene's TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process. The ORF cloned in this vector can be expressed as a tagged protein with a C-terminal monomeric GFP tag. P2A-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells. A brief lentiviral packaging protocol is provided. E. Coli selection: Chloramphenicol (34ug/ml) V2_F Primer can be used to sequence the target sequence after cloning. mGFP_R Primer can be used to sequence the target sequence after cloning.
Schematic of the multiple cloning sites

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Documents

Product Manuals
TrueORF and PrecisionShuttle cloning vectors Application Guide
FAQs
FAQ for Tagged Cloning Vectors
SDS for PS100093

Resources

Vector Resources
Video Tutorial: Clone Selection Guide Product Video: Why Should You Choose TrueORF GOLD shRNA Selection Guide Protocol: Lentivirus packaging CRISPR Knockout kit manual CRISPR vector manual CRISPR Trangene Knockin at AAVS1 and ROSA26 loci manual CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) manual AAV Biosafety

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

GE100111	Forward primer to sequence targets cloned in expression vectors.	1 nmol
GE100125	Reverse primer to sequence targets cloned in expression vectors.	1 nmol
TR30037	Lenti-vpak packaging kit - packaging plasmids and transfection reagent	10 reactions
TF81001	TurboFectin Transfection Reagent (1 mL in 1 vial)	1 ml in 1 vial

pLenti-C-mGFP-P2A-Puro Lentiviral Gene Expression Vector

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pLenti-C-mGFP-P2A-Puro Lentiviral Gene Expression Vector

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pLenti-C-mGFP-P2A-Puro Lentiviral Gene Expression Vector

Référence PS100093

Contactez votre distributeur local :

pLenti-C-mGFP-P2A-Puro Lentiviral Gene Expression Vector

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