Prion Protein, aa109-112 (PrP, CD230) (APC)

The prion protein is a large membrane protein that occurs normally in neurons of the human brain and is thought to be involved in synaptic transmission. In prion diseases, such as CJD, Gerstmann-Straussler-Scheinker syndrome (GSS), Fatal Familial Insomnia (FFI), Alpers Syndrome and Kuru, the normal cellular form of this protein (PrPc) is transformed into an altered protein when it comes into contact with an infectious prion protein (PrPsc) from another host. This altered PrPsc accumulates in cytoplasmic vesicles of diseased individuals forming lesions, vacuoles and amyloid deposits.||Applications:|Suitable for use in Immunohistochemistry, Western Blot, Immunoprecipitation and ELISA. Other applications have not been tested.||Recommended Dilution:|Immunohistochemistry (parrafin): 1:100-1:1000 Requires citrate buffer (HIER). Immunoreactivity is seen predominantly as cell body staining of neurons. Epitope must be re-exposed in fixed tissue by pretreatment of tissue using one of the following procedures: |a. formic acid for 10 minutes at RT |b. hydrolytic autoclaving |c. microwaving |Western Blot: 1:10,000-1:100,000|Immunoprecipitation: 1:10-1:100|ELISA: ≥ 1:100,000|Optimal dilutions to be determined by the researcher.||Recommended Control: |Brain tissue||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. ||Note: Applications are based on unconjugated antibody.