K1009-10 | Protease Inhibitor Cocktail (EDTA-Free, 100X in DMSO) Clinisciences

Product Citation

1. Yuze Yan, Miaomiao Tian, et al. "ASH1L haploinsufficiency results in autistic-like phenotypes in mice and links Eph receptor gene to autism spectrum disorder." Neuron. 2022 Jan 20;S0896-6273(21)01090-4. PMID: 35081333

Description

Endogenous proteins are produced and degraded in a balanced state, so their cellular levels are stable under stable environmental conditions. Crude cell extracts contain a number of endogenous enzymes, such as phosphatases and proteases, which are capable of degrading proteins in the extracts. Protein production is greatly halted and degradation is increased when proteins are extracted from cells and tissues in vitro. The best way to increase the yield of intact proteins is to add inhibitors of those enzymes known to be present.

Protease inhibitor cocktail is used with fungal and yeast extracts to increase protein stability. The cocktail functions to inhibit proteases that would degrade either non-phosphorylated or phosphorylated protein substrates.

His protease inhibitor cocktail contains individual components, including AEBSF, 1,10-Phenanthroline, E-64 and Pepstatin A with a broad specificity for cysteine, serine, aspartic, and metalloproteases. This cocktail has been optimized and tested for fungal and yeast cell use. This protease inhibitor cocktail is supplied as a ready-to-use solution in DMSO.

Quality Control

DataSheet

Protocol

Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as yeast extracts) before assaying.

Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.

Components and Storage

Catalog No.	Product Name	Summary	Targets	CAS Number	Smiles
B7854	o-Phenanthroline	metalloprotease inhibitor	Proteases\|Other Proteases	66-71-7	C(C=CC=N1)(C=C2)=C1C3=C2C=CC=N3
A2573	AEBSF.HCl	Serine protease inhibitor	Proteases\|Serine Protease	30827-99-7	C1=CC(=CC=C1CCN)S(=O)(=O)F.Cl
A2576	E-64	Cysteine protease inhibitor,irriversible	Proteases\|Cathepsin	26305-03-3	CC(C)CC(C(CC(=O)O)O)NC(=O)C(C)NC(=O)CC(C(CC(C)C)NC(=O)C(C(C)C)NC(=O)C(C(C)C)NC(=O)CC(C)C)O
A2571	Pepstatin A	Aspartic proteinases inhibitor	Proteases\|Other Proteases	103476-89-7	O=C(C(C(16)(16)C(C(16)(16)16)(16)C(16)(16)16)(16)N(16)C(C(16)(16)16)=O)N(16)C(C(N(16)C(C(16)=O)(16)C(16)(16)C(16)(16)C(16)(16)/N=C(N(16)16)/N(16)16)=O)(16)C(16)(16)C(C(16)(16)16)(16)C(16)(16)16
Stored at -20°C, and stable for at least 12 months.

Storage Conditions

Stored at -20°C, and stable for at least 12 months.

Protease Inhibitor Cocktail (EDTA-Free, 100X in DMSO)

Référence K1009-10

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