K1010-50 | Protease Inhibitor Cocktail (EDTA-Free, 100X in DMSO) Clinisciences

Product Citation

1. Lior Zimmerman, Noga Alon, et al. "Context-Dependent Design of Induced-fit Enzymes using Deep Learning Generates Well Expressed, Thermally Stable and Active Enzymes." Proc Natl Acad Sci U S A. 2024 Mar 12;121(11):e2313809121. PMID: 38437538
2. Qing Zhong, et al. "Human YKT6 forms priming complex with STX17 and SNAP29 to facilitate autophagosome-lysosome fusion." Cell Rep. 2024 Feb 27;43(2):113760. PMID: 38340317
3. Shuyao Hu 1, Yufeng Zhang 2, et al. "Time-resolved proteomic profiling reveals compositional and functional transitions across the stress granule life cycle." Nat Commun. 2023 Nov 27;14(1):7782. PMID: 38012130
4. Yuhui Fu, Binbin Ding, et al. "Qa-SNARE syntaxin 18 mediates lipid droplet fusion with SNAP23 and SEC22B." Cell Discov. 2023 Nov 21;9(1):115. PMID: 37989733
5. Hong-Wei Zhang, Kun Huang, et al. "A cryo-EM structure of KTF1-bound polymerase V transcription elongation complex." Nat Commun. 2023 May 30;14(1):3118. PMID: 37253723
6. Jin-Peng Gao, Wenjie Liang, et al. "NODULE INCEPTION activates gibberellin biosynthesis genes during rhizobial infection." New Phytol. 2023 Jan 22. PMID: 36683391

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Quality Control

DataSheet

Protocol

Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as cell lysates) before assaying.

Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.

Components and Storage

Catalog No.	Product Name	Summary	Targets	CAS Number	Smiles
A2573	AEBSF.HCl	Serine protease inhibitor	Proteases\|Serine Protease	30827-99-7	C1=CC(=CC=C1CCN)S(=O)(=O)F.Cl
A2575	Bestatin	Aminopeptidase inhibitor	Proteases\|Aminopeptidase	58970-76-6	CC(C)CC(C(=O)O)NC(=O)C(C(CC1=CC=CC=C1)N)O
A2576	E-64	Cysteine protease inhibitor,irriversible	Proteases\|Cathepsin	66701-25-5	CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O
A2571	Pepstatin A	Aspartic protease inhibitor	Proteases\|Other Proteases	26305-03-3	CC(C)CC(C(CC(=O)O)O)NC(=O)C(C)NC(=O)CC(C(CC(C)C)NC(=O)C(C(C)C)NC(=O)C(C(C)C)NC(=O)CC(C)C)O
B4790	Phosphoramidon Disodium Salt	metalloproteinase inhibitor		164204-38-0	CC(C[C@@](NP(O)(O[C@@]1(23)[C@@](O)(23)[C@@](O)(23)[C@](O)(23)[C@](O1)(23)C)=O)(23)/C([O-])=N/[C@@](C([O-])=O)(23)CC2=CNC3=CC=CC=C23)C.[Na+].[Na+]
Stored at -20°C, and stable for at least 12 months.

Description

Endogenous proteins are produced and degraded in a balanced state, so their cellular levels are stable under stable environmental conditions. Crude cell extracts contain a number of endogenous enzymes, such as phosphatases and proteases, which are capable of degrading proteins in the extracts. Protein production is greatly halted and degradation is increased when proteins are extracted from cells and tissues in vitro. The best way to increase the yield of intact proteins is to add inhibitors of those enzymes known to be present.

Protease inhibitor cocktail is used in purification of Histidine-tagged proteins to increase protein stability. The cocktail functions to inhibit proteases that would degrade either non-phosphorylated or phosphorylated protein substrates.

This protease inhibitor cocktail contains individual components, including AEBSF, Bestatin, E-64, Phosphoramidon and Pepstatin A with a broad specificity for cysteine, serine, aspartic, and thermolysin-like proteases, and aminopeptidases. This protease inhibitor cocktail has been optimized and tested for purification of histidine-tagged proteins. This protease inhibitor cocktail is supplied as a ready-to-use solution in DMSO.

Storage

Stored at -20°C, and stable for at least 12 months.

Protease Inhibitor Cocktail (EDTA-Free, 100X in DMSO)

Référence K1010-50

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