PSD95 Antibody
Référence OASE00034
Conditionnement : 25ug
Marque : Aviva Systems Biology
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Téléphone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for OASE00034 |
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| Predicted Species Reactivity | Human, Mouse, Rat, Bovine |
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| Product Format | Liquid PBS (pH 7.4) with 50% glycerol and 0.09% sodium azide |
| Clonality | Monoclonal |
| Clone | 7E3 |
| Isotype | IgG1 |
| Host | Mouse |
| Conjugation | Unconjugated |
| Application | WB, IHC, ICC, IF, AM |
| :: | Certificate of Analysis: 1ug/ml was sufficient for detection of PSD-95 on 20ug rat brain tissue extract by ECL immunoblot analysis using Goat Anti-Mouse IgG: HRP as the secondary. Cellular Localization: Cell membrane, cell junction, synapse, postsynaptic cell membrane, postsynaptic density, cell projection, axon. |
| Reconstitution and Storage | Store at -20C. Shipping with Blue Ice or 4C. |
| Immunogen | Recombinant rat PSD-95 |
| Purification | Protein G Purified |
| Concentration | 1 mg/ml |
| Specificity | Detects ~100kDa. An additional protein of >100kDa is also detected. Additional cross-reactive bands are detected at ~75kDa and 50kDa in rat and mouse samples. |
| Dilution | WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user. |
| Application Info | WB: 1:1000 IHC: 1:1000 ICC/IF: 1:100 |
| Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
| Description | Postsynaptic Density protein 95 (PSD95), also known as Synapse associated protein 90kDa, is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein and is involved in the assembly and function of the postsynaptic density complex (1). These family members consist of an N-terminal variable segment followed by three amino-terminal PDZ domains, an upstream SH3 domain and an inactive carboxyl-terminal guanylate kinase (GK) domain. The first and second PDZ domain localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins which are neuronal cell adhesion molecules that interact with b-neurexins and form intercellular junctions. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interactions between PDZ domains present on both proteins (2). Thus different PDZ domains of PSD-95 might be specialized for distinct functions (3, 4). PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs) (5). The protein is implicated in experience dependent plasticity and plays an indispensable role in learning (6). Mutations in PSD95 are associated with autism (7). |
| Reference | 1. Chetkovich D.M., Bunn R.C., Kuo S.H., Kawasaki Y., Kohwi M., and Bredt D.S. (2002) J Neurosci. 22(15): 6415-25. 2. Cao J., Viholainen J.I., Dart C., Warwick H.K., Levland M.L. and Courtney M.J. (2005) J Cell Biol. 168(1): 117-26. 3. Kennedy M. (1997) Trends in Neurosci. 6: 264-268. 4. Irie M. et al. (1997) Science 277(5331): 1511-5. 5. Cai C. et al. (2006) J Biol Chem. 281: 4267-73. 6. Yao W.D. et al. (2004) Neuron 41: 625-38. 7. Cline H. (2005) Curr Biol. 15: R203-5. |
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| Gene Symbol | PSD95 |
| Gene Full Name | discs, large homolog 4 (Drosophila) |
| Alias Symbols | PSD 95, PSD-95, DLG4, SAP90, Synapse-associated protein 90, Postsynaptic density protein 95, Disks large homolog 4 |
| NCBI Gene Id | 29495 |
| Description of Target | Postsynaptic Density protein 95 (PSD95), also known as Synapse associated protein 90kDa, is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein and is involved in the assembly and function of the postsynaptic density complex. These family members consist of an N-terminal variable segment followed by three amino-terminal PDZ domains, an upstream SH3 domain and an inactive carboxyl-terminal guanylate kinase (GK) domain. The first and second PDZ domain localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins which are neuronal cell adhesion molecules that interact with b-neurexins and form intercellular junctions. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interactions between PDZ domains present on both proteins. Thus different PDZ domains of PSD-95 might be specialized for distinct functions. PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs). The protein is implicated in experience dependent plasticity and plays an indispensable role in learning. Mutations in PSD95 are associated with autism. |
| Uniprot ID | P31016 |
| Protein Accession # | NP_062567.1 |
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What is the species homology for "PSD95 Antibody (OASE00034)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Bovine".
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How long will it take to receive "PSD95 Antibody (OASE00034)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "PSD95 Antibody (OASE00034)" provided in?
This item is provided in "Liquid PBS (pH 7.4) with 50% glycerol and 0.09% sodium azide".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "PSD95 Antibody (OASE00034)"?
This target may also be called "PSD 95, PSD-95, DLG4, SAP90, Synapse-associated protein 90, Postsynaptic density protein 95, Disks large homolog 4" in publications.
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What is the shipping cost for "PSD95 Antibody (OASE00034)"?
The shipping cost for this item is within the US. Please contact us for specific shipping for international orders.
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What is the guarantee for "PSD95 Antibody (OASE00034)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "PSD95 Antibody (OASE00034)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "PSD95 Antibody (OASE00034)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "PSD95"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "PSD95"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "PSD95"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "PSD95"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "PSD95"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "PSD95"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.