PTPN22 (NM_015967) Human Tagged ORF Clone
Référence RC218969
Conditionnement : 10µg
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PTPN22 (NM_015967) Human Tagged ORF Clone
SKU
RC218969
PTPN22 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, non-receptor type 22 (lymphoid) (PTPN22), transcript variant 1
| Product Data | |
| Target Symbol | PTPN22 |
|---|---|
| Synonyms | LYP; LYP1; LYP2; PEP; PTPN8; PTPN22.5; PTPN22.6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | ORF Nucleotide Sequence >RC218969 representing NM_015967. Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGACCAAAGAGAAATTCTGCAGAAGTTCCTGGATGAGGCCCAAAGCAAGAAAATTACTAAAGAGGAG TTTGCCAATGAATTTCTGAAGCTGAAAAGGCAATCTACCAAGTACAAGGCAGACAAAACCTATCCTACA ACTGTGGCTGAGAAGCCCAAGAATATCAAGAAAAACAGATATAAGGATATTTTGCCCTATGATTATAGC CGGGTAGAACTATCCCTGATAACCTCTGATGAGGATTCCAGCTACATCAATGCCAACTTCATTAAGGGA GTTTATGGACCCAAGGCTTATATTGCCACCCAGGGTCCTTTATCTACAACCCTCCTGGACTTCTGGAGG ATGATTTGGGAATATAGTGTCCTTATCATTGTTATGGCATGCATGGAGTATGAAATGGGAAAGAAAAAG TGTGAGCGCTACTGGGCTGAGCCAGGAGAGATGCAGCTGGAATTTGGCCCTTTCTCTGTATCCTGTGAA GCTGAAAAAAGGAAATCTGATTATATAATCAGGACTCTAAAAGTTAAGTTCAATAGTGAAACTCGAACT ATCTACCAGTTTCATTACAAGAATTGGCCAGACCATGATGTACCTTCATCTATAGACCCTATTCTTGAG CTCATCTGGGATGTACGTTGTTACCAAGAGGATGACAGTGTTCCCATATGCATTCACTGCAGTGCTGGC TGTGGAAGGACTGGTGTTATTTGTGCTATTGATTATACATGGATGTTGCTAAAAGATGGGATAATTCCT GAGAACTTCAGTGTTTTCAGTTTGATCCGGGAAATGCGGACACAGAGGCCTTCATTAGTTCAAACGCAG GAACAATATGAACTGGTCTACAATGCTGTATTAGAACTATTTAAGAGACAGATGGATGTTATCAGAGAT AAACATTCTGGAACAGAGAGTCAAGCAAAGCATTGTATTCCTGAGAAAAATCACACTCTCCAAGCAGAC TCTTATTCTCCTAATTTACCAAAAAGTACCACAAAAGCAGCAAAAATGATGAACCAACAAAGGACAAAA ATGGAAATCAAAGAATCTTCTTCCTTTGACTTTAGGACTTCTGAAATAAGTGCAAAAGAAGAGCTAGTT TTGCACCCTGCTAAATCAAGCACTTCTTTTGACTTTCTGGAGCTAAATTACAGTTTTGACAAAAATGCT GACACAACCATGAAATGGCAGACAAAGGCATTTCCAATAGTTGGGGAGCCTCTTCAGAAGCATCAAAGT TTGGATTTGGGCTCTCTTTTGTTTGAGGGATGTTCTAATTCTAAACCTGTAAATGCAGCAGGAAGATAT TTTAATTCAAAGGTGCCAATAACACGGACCAAATCAACTCCTTTTGAATTGATACAGCAGAGAGAAACC AAGGAGGTGGACAGCAAGGAAAACTTTTCTTATTTGGAATCTCAACCACATGATTCTTGTTTTGTAGAG ATGCAGGCTCAAAAAGTAATGCATGTTTCTTCAGCAGAACTGAATTATTCACTGCCATATGACTCTAAA CACCAAATACGTAATGCCTCTAATGTAAAGCACCATGACTCTAGTGCTCTTGGTGTATATTCTTACATA CCTTTAGTGGAAAATCCTTATTTTTCATCATGGCCTCCAAGTGGTACCAGTTCTAAGATGTCTCTTGAT TTACCTGAGAAGCAAGATGGAACTGTTTTTCCTTCTTCTCTGTTGCCAACATCCTCTACATCCCTCTTC TCTTATTACAATTCACATGATTCTTTATCACTGAATTCTCCAACCAATATTTCCTCACTATTGAACCAG GAGTCAGCTGTACTAGCAACTGCTCCAAGGATAGATGATGAAATCCCCCCTCCACTTCCTGTACGGACA CCTGAATCATTTATTGTGGTTGAGGAAGCTGGAGAATTCTCACCAAATGTTCCCAAATCCTTATCCTCA GCTGTGAAGGTAAAAATTGGAACATCACTGGAATGGGGTGGAACATCTGAACCAAAGAAATTTGATGAC TCTGTGATACTTAGACCAAGCAAGAGTGTAAAACTCCGAAGTCCTAAATCAGAACTACATCAAGATCGT TCTTCTCCCCCACCTCCTCTCCCAGAAAGAACTCTAGAGTCCTTCTTTCTTGCCGATGAAGATTGTATG CAGGCCCAATCTATAGAAACATATTCTACTAGCTATCCTGACACCATGGAAAATTCAACATCTTCAAAA CAGACACTGAAGACTCCTGGAAAAAGTTTCACAAGGAGTAAGAGTTTGAAAATTTTGCGAAACATGAAA AAGAGTATCTGTAATTCTTGCCCACCAAACAAGCCTGCAGAATCTGTTCAGTCAAATAACTCCAGCTCA TTTCTGAATTTTGGTTTTGCAAACCGTTTTTCAAAACCCAAAGGACCAAGGAATCCACCACCAACTTGG AATATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC Protein Sequence >Peptide sequence encoded by RC218969 Blue=ORF Red=Cloning site Green=Tag(s) MDQREILQKFLDEAQSKKITKEEFANEFLKLKRQSTKYKADKTYPTTVAEKPKNIKKNRYKDILPYDYS RVELSLITSDEDSSYINANFIKGVYGPKAYIATQGPLSTTLLDFWRMIWEYSVLIIVMACMEYEMGKKK CERYWAEPGEMQLEFGPFSVSCEAEKRKSDYIIRTLKVKFNSETRTIYQFHYKNWPDHDVPSSIDPILE LIWDVRCYQEDDSVPICIHCSAGCGRTGVICAIDYTWMLLKDGIIPENFSVFSLIREMRTQRPSLVQTQ EQYELVYNAVLELFKRQMDVIRDKHSGTESQAKHCIPEKNHTLQADSYSPNLPKSTTKAAKMMNQQRTK MEIKESSSFDFRTSEISAKEELVLHPAKSSTSFDFLELNYSFDKNADTTMKWQTKAFPIVGEPLQKHQS LDLGSLLFEGCSNSKPVNAAGRYFNSKVPITRTKSTPFELIQQRETKEVDSKENFSYLESQPHDSCFVE MQAQKVMHVSSAELNYSLPYDSKHQIRNASNVKHHDSSALGVYSYIPLVENPYFSSWPPSGTSSKMSLD LPEKQDGTVFPSSLLPTSSTSLFSYYNSHDSLSLNSPTNISSLLNQESAVLATAPRIDDEIPPPLPVRT PESFIVVEEAGEFSPNVPKSLSSAVKVKIGTSLEWGGTSEPKKFDDSVILRPSKSVKLRSPKSELHQDR SSPPPPLPERTLESFFLADEDCMQAQSIETYSTSYPDTMENSTSSKQTLKTPGKSFTRSKSLKILRNMK KSICNSCPPNKPAESVQSNNSSSFLNFGFANRFSKPKGPRNPPPTWNI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms | Chromatograms  Sequencher program is needed, download here |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map  |
| ACCN | NM_015967 |
| ORF Size | 2421 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_015967.7 |
| RefSeq Size | 3615 bp |
| RefSeq ORF | 2424 bp |
| Locus ID | 26191 |
| UniProt ID | Q9Y2R2 |
| Cytogenetics | 1p13.2 |
| Domains | PTPc_motif, Y_phosphatase |
| Protein Families | Druggable Genome, Phosphatase |
| MW | 91.7 kDa |
| Summary | This gene encodes of member of the non-receptor class 4 subfamily of the protein-tyrosine phosphatase family. The encoded protein is a lymphoid-specific intracellular phosphatase that associates with the molecular adapter protein CBL and may be involved in regulating CBL function in the T-cell receptor signaling pathway. Mutations in this gene may be associated with a range of autoimmune disorders including Type 1 Diabetes, rheumatoid arthritis, systemic lupus erythematosus and Graves' disease. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Mar 2009] |
| Product Manuals |
| FAQs |
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| SDS |
| SKU | Description | Size | ||
|---|---|---|---|---|
| RC218969L1 | Lenti ORF clone of Human protein tyrosine phosphatase, non-receptor type 22 (lymphoid) (PTPN22), transcript variant 1, Myc-DDK-tagged | 10 ug | | |
| RC218969L2 | Lenti ORF clone of Human protein tyrosine phosphatase, non-receptor type 22 (lymphoid) (PTPN22), transcript variant 1, mGFP tagged | 10 ug | | |
| RC218969L3 | Lenti ORF clone of Human protein tyrosine phosphatase, non-receptor type 22 (lymphoid) (PTPN22), transcript variant 1, Myc-DDK-tagged | 10 ug | | |
| RC218969L4 | Lenti ORF clone of Human protein tyrosine phosphatase, non-receptor type 22 (lymphoid) (PTPN22), transcript variant 1, mGFP tagged | 10 ug | | |
| RG218969 | PTPN22 (tGFP-tagged) - Human protein tyrosine phosphatase, non-receptor type 22 (lymphoid) (PTPN22), transcript variant 1 | 10 ug | | |
| SC114550 | PTPN22 (untagged)-Human protein tyrosine phosphatase, non-receptor type 22 (lymphoid) (PTPN22), transcript variant 1 | 10 ug | | |
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