Rabbit polyclonal antibody to Vinculin
Référence CPA4307-200ul
Conditionnement : 200ul
Marque : Cohesion Biosciences
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- Western blot analysis of Vinculin expression in PC12 (A), HepG2 (B), Hela (C) whole cell lysates.
- Immunohistochemical analysis of Vinculin staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
- Immunofluorescent analysis of Vinculin staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
| Product Name | Anti-Vinculin Antibody |
| Cat No: | CPA4307 |
| Source: | Rabbit |
| Reactivity: | H, M, R, C, P |
| Applications: | WB, IH, IF/IC |
| *Application Key: | |
| E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference | |
| *Species Reactivity Key: | |
| H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish | |
Description: Rabbit polyclonal antibody to Vinculin Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Vinculin. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) Gene Symbol: VCL Alternative Names: Vinculin; Metavinculin; MVEntrez Gene (Human): Entrez Gene (Mouse) : Entrez Gene (Rat) : SwissProt (Human): SwissProt (Mouse) : SwissProt (Rat) : Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.