General Info
| Applications: | ELISA |
| Reactivity: | Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.064ng/mL |
| Detection Limit: | 0.156-10ng/mL |
| Short Description: | This CYP11A1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 3 hrs |
Information
| Gene Symbol: | Cyp11a1 |
| Gene ID: | 29680 |
| Uniprot ID: | CP11A_RAT |
| Immunogen Region: | Ready-To-Use |
| Sample Type: | tissue homogenates or other biological fluids. |
Description
| Tissue Specificity | Expressed in the kidney where it localizes to the distal convoluted tubule and the thick ascending limb of the loop of Henle (at protein level). In the ovary, highly expressed in interstitial cells (at protein level). Also expressed in adrenal gland and testis. |
| Post Translational Modifications | |
| Function | A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones. Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin). |
| Protein Name | Cholesterol Side-Chain Cleavage Enzyme - MitochondrialCypxia1Cholesterol DesmolaseCytochrome P450 11a1Cytochrome P450(Scc |
| Database Links | Reactome: R-RNO-196108Reactome: -RNO-211976 |
| Cellular Localisation | Mitochondrion Inner MembranePeripheral Membrane ProteinLocalizes To The Matrix Side Of The Mitochondrion Inner Membrane |
| Alternative ELISA Names | Cholesterol Side-Chain Cleavage Enzyme - Mitochondrial ELISA kitCypxia1 ELISA kitCholesterol Desmolase ELISA kitCytochrome P450 11a1 ELISA kitCytochrome P450(Scc ELISA kitCyp11a1 ELISA kitCyp11a ELISA kitCyp11a-1 ELISA kit |
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Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance