Rat HSPA8 (Heat Shock 70kDa Protein 8) Sandwich ELISA Kit

Storage Instruction:	Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	4.5 hrs

Gene Symbol:	Hspa8
Gene ID:	24468
Uniprot ID:	HSP7C_RAT
Sample Type:	serum, plasma or other biological fluids.

Tissue Specificity
Post Translational Modifications	Acetylated. ISGylated. Trimethylation at Lys-561 reduces fibrillar SNCA binding.
Function	Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, chaperone-mediated autophagy, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The co-chaperones have been shown to not only regulate different steps of the ATPase cycle of HSP70, but they also have an individual specificity such that one co-chaperone may promote folding of a substrate while another may promote degradation. The affinity of HSP70 for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. HSP70 goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. The HSP70-associated co-chaperones are of three types: J-domain co-chaperones HSP40s , the nucleotide exchange factors (NEF) such as BAG1/2/3 (facilitate conversion of HSP70 from the ADP-bound to the ATP-bound state thereby promoting substrate release), and the TPR domain chaperones such as HOPX and STUB1. Plays a critical role in mitochondrial import, delivers preproteins to the mitochondrial import receptor TOMM70. Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Substrate recognition component in chaperone-mediated autophagy (CMA), a selective protein degradation process that mediates degradation of proteins with a -KFERQ motif: HSPA8/HSC70 specifically recognizes and binds cytosolic proteins bearing a -KFERQ motif and promotes their recruitment to the surface of the lysosome where they bind to lysosomal protein LAMP2. KFERQ motif-containing proteins are eventually transported into the lysosomal lumen where they are degraded. Participates in the ER-associated degradation (ERAD) quality control pathway in conjunction with J domain-containing co-chaperones and the E3 ligase STUB1. Interacts with VGF-derived peptide TLQP-21.
Protein Name	Heat Shock Cognate 71 Kda Protein Heat Shock 70 Kda Protein 8
Database Links	Reactome: R-RNO-3371453 Reactome: -RNO-3371497 Reactome: -RNO-3371568 Reactome: -RNO-3371571 Reactome: -RNO-432720 Reactome: -RNO-432722 Reactome: -RNO-450408 Reactome: -RNO-6798695 Reactome: -RNO-72163 Reactome: -RNO-8856828 Reactome: -RNO-8876725 Reactome: -RNO-888590
Cellular Localisation	Cytoplasm Melanosome Nucleus Nucleolus Cell Membrane Lysosome Membrane Peripheral Membrane Protein Cytoplasmic Side Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas Translocates Rapidly From The Cytoplasm To The Nuclei And Especially To The Nucleoli Upon Heat Shock
Alternative ELISA Names	Heat Shock Cognate 71 Kda Protein ELISA kit Heat Shock 70 Kda Protein 8 ELISA kit Hspa8 ELISA kit Hsc70 ELISA kit Hsc73 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance