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> RayPlex Human Cell Death Bead Array 1

RayPlex Human Cell Death Bead Array 1

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Référence FAH-CD-1-96

Conditionnement : 96tests

Marque : RayBiotech

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RayPlex Human Cell Death Bead Array 1

This RayPlex® kit is a cytometric bead array to quantitatively measure, by flow cytometry, 13 key targets involved in cell death pathways. The assay has been optimized for serum, plasma, and cell culture supernatant.

Product Description

Specifications

Size 192 tests, 48 tests, 480 tests, 96 tests
Species Human
Quantitative/Semi-Quantitative Quantitative
Number of Targets Detected 13
Compatible Sample Types Cell Culture Supernatants, Plasma, Serum
Solid Support Fluorescent Microbeads
Method Of Detection Flow Cytometry
Design Principle Sandwich-based
Estimated Lead Time 1-2 business days
Shipping Type Blue ice
Storage 4°C

Product Features

  • Less sample, more data: up to 25 proteins can be quantified from only 25 µl or less of your precious sample
  • 4 hour processing time
  • 5-10x more cost-effective than ELISA
  • Compatible with most flow cytometers
  • No specialized or dedicated instrument(s) needed
  • High specificity and system reproducibility
  • Well-suited for high throughput assays

Target Names

Scroll over each target protein for more information
Bcl-2
  • Gene Symbol: BCL2
  • Gene ID: 596
  • Accession #:
  • ELISA Kit:
BID
  • Gene Symbol: BID
  • Gene ID: 637
  • Accession #:
  • ELISA Kit:
Caspase-3
  • Gene Symbol: CASP3 CPP32
  • Gene ID: 836
  • Accession #:
  • ELISA Kit: RayBio
CD40
(TNFRSF5)
  • Gene Symbol: CD40 TNFRSF5
  • Gene ID: 958
  • Accession #:
  • ELISA Kit:
CD40 Ligand
(TNFSF5)
  • Gene Symbol: CD40LG CD40L TNFSF5 TRAP
  • Gene ID: 959
  • Accession #:
  • ELISA Kit:
Fas
(TNFRSF6/Apo-1)
  • Gene Symbol: FAS APT1 FAS1 TNFRSF6
  • Gene ID: 355
  • Accession #:
  • ELISA Kit:
Fas Ligand
(TNFSF6)
  • Gene Symbol: FASLG APT1LG1 CD95L FASL TNFSF6
  • Gene ID: 356
  • Accession #:
  • ELISA Kit:
TNF alpha
  • Gene Symbol: TNF TNFA TNFSF2
  • Gene ID: 7124
  • Accession #:
  • ELISA Kit:
TNF beta
(TNFSF1B)
  • Gene Symbol: LTA TNFB TNFSF1
  • Gene ID: 4049
  • Accession #:
  • ELISA Kit:
TNF RI
(TNFRSF1A)
  • Gene Symbol: TNFRSF1A TNFAR TNFR1
  • Gene ID: 7132
  • Accession #:
  • ELISA Kit:
TNF RII
(TNFRSF1B)
  • Gene Symbol: TNFRSF1B TNFBR TNFR2
  • Gene ID: 7133
  • Accession #:
  • ELISA Kit:
TRAIL R1
(TNFRSF10A/DR4)
  • Gene Symbol: TNFRSF10A APO2 DR4 TRAILR1
  • Gene ID: 8797
  • Accession #:
  • ELISA Kit:
TRAIL R2
(TNFRSF10B/DR5)
  • Gene Symbol: TNFRSF10B DR5 KILLER TRAILR2 TRICK2 ZTNFR9 UNQ160/PRO186
  • Gene ID: 8795
  • Accession #:
  • ELISA Kit:
   

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • RayPlex™ Multiplex Bead Cocktail
  • 1X Assay Diluent
  • 20X Wash Buffer
  • RayBio® Serum/Plasma Diluent
  • Lyophilized Protein Standard Mix
  • 1X Biotinylated Detection Antibody Cocktail
  • 10X Streptavidin-PE
  • Flow Cytometer Setup Bead Cocktail
  • V-bottom 96-well Microplate
  • Filter 96-well Microplate
Other Materials Required
  • Orbital 96-well plate shaker (with ability to reach 1000 rpm)
  • Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
  • Aluminum foil or 96-well Plate Aluminum Sealers
  • Distilled water
  • 1.5 ml polypropylene microcentrifuge tubes or similar
  • Microcentrifuge
  • If using the Filter 96-Well Microplate (Item 11): Vacuum manifold compatible with a 96-well microplate
  • Optional: 96-well round bottom plate (to prepare samples)
  • Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. !

Protocol Outline
  1. Prepare Reagents, Standards, and Samples
  2. Add RayPlex™ Multiplex Bead Cocktail to each well
  3. Incubate with Samples and Standards
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluorophore
  6. Analyze with a compatible flow cytometer
  7. Perform data analysis

Storage/Stability

Upon receipt, all components should be stored at 4°C. The Lyophilized Protein Standard Mix (Item 4) should be stored at 4°C or below. Use reagents immediately after preparation. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.