Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 18-141aa
- UniProt No.
- P01587
- NCBI Accession No.
- NP_034099
- Alternative names
- Granulocyte-macrophage colony stimulating factor 2, Csf2, Csfgm, MGI-IGM, mGM-CSF, Granulocyte-macrophage colony stimulating factor 2, Granulocyte-macrophage colony stimulating factor 2
Product Specification
- Molecular Weight
- 14 kDa (125aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) 10% glycerol
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Measured in a cell proliferation assay using MC/9 mouse mast cells. The ED50 for this effect is less or equal to 0.06ng/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- Non-Tagged
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Biological Activity
Mouse GM-CSF stimulates cell proliferation of the MC/9 mouse mast cells. The ED50 range is less or equal 0.06 ng/ml.
Related Publications
- Hotta M, et al. GM-CSF therapy inhibits chronic graft-versus-host disease via expansion of regulatory T cells. Eur J Immunol. 2019 Jan;49(1):179-191.
Tsubokura Y, et al. Early administration of lenalidomide after allogeneic hematopoietic stem cell transplantation suppresses graft-versus-host disease by inhibiting T-cell migration to the gastrointestinal tract. (Immun Inflamm Dis. 2022 Sep)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.