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> Resazurin (sodium) [62758-13-8]

Resazurin (sodium) [62758-13-8]

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Resazurin sodium (Diazoresorcinol sodium) is commonly used to measure bacterial and eukaryotic cell viability through its reduction to the fluorescent product resorufin.

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Resazurin sodium Chemical Structure

Resazurin sodium Chemical Structure

CAS No. : 62758-13-8

This product is a controlled substance and not for sale in your territory.

Based on 3 publication(s) in Google Scholar

Other Forms of Resazurin sodium:

  • Resazurin Obtenir un devis
  • Resazurin sodium (Standard) Obtenir un devis
Description

Resazurin sodium (Diazoresorcinol sodium) is commonly used to measure bacterial and eukaryotic cell viability through its reduction to the fluorescent product resorufin.

In Vitro

Resazurin sodium (Diazoresorcinol sodium) is commonly used to measure bacterial and eukaryotic cell viability through its reduction to the fluorescent product resorufin. No viable bacteria are detected 24 h post-inoculation following inclusion of Resazurin sodium in TSBc cultures of F. tularensis LVS at the concentration of 44 μM. Lowering the Resazurin sodium concentration to as little as 4.4 μM still results in a 10-fold reduction in viable F. tularensis LVS compare to growth medium alone. Both Resazurin sodium treatments result in a significant decrease in viable F. tularensis LVS bacteria over 22 h. Treatment with Resazurin sodium significantly reduces the number of viable F. tularensis LVS bacteria in HEK293 cells 22 h post-infection[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Resazurin sodium Related Antibodies
Masse moléculaire

251.17

Formule

C12H6NNaO4
CAS No.

62758-13-8
Appearance

Solid

Color

Light brown to black

SMILES

O=C1C=CC2=[N+]([O-])C3=C(C=C(O[Na])C=C3)OC2=C1
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvant et solubilité
In Vitro: 

H2O : 5 mg/mL (19.91 mM; ultrasonic and warming and heat to 60°C)

DMSO : 2 mg/mL (7.96 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.9814 mL 19.9068 mL 39.8137 mL
5 mM 0.7963 mL 3.9814 mL 7.9627 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Calculateur de molarité

  • Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (8.28 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (8.28 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation

Purity: ≥98.0%

COA (248 KB) HNMR (205 KB)

Instruction de manipulation (2659 KB)
Références

  • [1]. Schmitt DM, et al. The use of resazurin as a novel antimicrobial agent against Francisella tularensis. Front Cell Infect Microbiol. 2013 Dec 6;3:93.  [Content Brief]

Test cellulaire
[1]

F. tularensis is cultured in TSBc supplemented with 44 μM Resazurin sodium salt at 37°C with shaking for 24 h. At select time points, a Spectronic 200 Spectrophotometer is used to measure the absorbance at 600 nm and 570 nm to detect the presence of Resazurin sodium salt and resorufin, respectively. The ratio of these two optical densities is used to evaluate reduction of Resazurin sodium salt to resorufin over time[1].

MCE n'a pas confirmé de manière indépendante l'exactitude de ces méthodes. Ils sont pour référence seulement.
Références

  • [1]. Schmitt DM, et al. The use of resazurin as a novel antimicrobial agent against Francisella tularensis. Front Cell Infect Microbiol. 2013 Dec 6;3:93.  [Content Brief]

  • [1]. Schmitt DM, et al. The use of resazurin as a novel antimicrobial agent against Francisella tularensis. Front Cell Infect Microbiol. 2013 Dec 6;3:93.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 3.9814 mL 19.9068 mL 39.8137 mL 99.5342 mL
5 mM 0.7963 mL 3.9814 mL 7.9627 mL 19.9068 mL
H2O 10 mM 0.3981 mL 1.9907 mL 3.9814 mL 9.9534 mL
15 mM 0.2654 mL 1.3271 mL 2.6542 mL 6.6356 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Resazurin sodium Related Classifications

  • Others
  • Anti-infection
  • Bacterial
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Resazurin62758-13-8DiazoresorcinolBacterialInhibitorinhibitorinhibit

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