RuBisCO ELISA Kit (Plant)
Référence OKCA00374
Conditionnement : 96Wells
Marque : Aviva Systems Biology
Predicted Species Reactivity | Plant | ||||||||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||||
ELISA Kit Duration | < 4 Hours | ||||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology RuBisCO ELISA Kit (Plant) (OKCA00374) is based on a competitive enzyme immuno assay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for RuBisCO and Horseradish Peroxidase (HRP) conjugated RuBisCO. The competitive inhibition reaction is launched between with HRP labeled RuBisCO and unlabeled RuBisCO with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of RuBisCO in the sample. The color development is stopped and the intensity of the color is measured. | ||||||||||||||||||||||||
ELISA Kit Range | 3.13 ug/mL - 800 ug/mL | ||||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Mean Intra-Assay Precision %CV: < 10% (n = 20) Mean Inter-Assay Precision %CV: < 20% (n = 20) | ||||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | CAS#: 9027-23-0 | ||||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||||
Sample Type | Plant tissues | ||||||||||||||||||||||||
Sensitivity | < 3.13 ug/mL | ||||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of plant RuBisCO. No significant cross-reactivity or interference between plant RuBisCO and analogues was observed. | ||||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive Immunoassay |
Gene Symbol | RuBisCO |
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Protein Name | RuBisCO |