S100B ELISA Kit (Human)
Référence OKEH02888
Conditionnement : 96Wells
Marque : Aviva Systems Biology
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| Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology S100B ELISA Kit (Human) (OKEH02888) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for S100B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for S100B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample S100B captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 15.6-1000pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | <= 6.4% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 3.9% (n = 20) Mean Inter-assay CV%: < 6.7% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 7.8 pg/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Human Protein S100-B | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | S100B |
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| Gene Full Name | S100 calcium binding protein B |
| Alias Symbols | NEF;protein S100-B;S100;S100 calcium-binding protein B;S100 calcium-binding protein, beta (neural);S-100 calcium-binding protein, beta chain;S-100 protein beta chain;S-100 protein subunit beta;S100-B;S100beta. |
| NCBI Gene Id | 6285 |
| Protein Name | P04271 |
| Description of Target | Protein S100-B |
| Uniprot ID | https://www.uniprot.org/uniprot/P04271 |
| Protein Accession # | NP_006263.1 |
| Nucleotide Accession # | NM_006272.2 |
| Protein Size (# AA) | 92 |
| Molecular Weight | 10 kDa |