Sarcoma-180
Référence 400330
Conditionnement : 1cryovial
Marque : CLS Cell Lines Service
Meth A sarcoma Cells
General information
Description | Meth A sarcoma cells have been established as in vitro cell line from the transplantable Meth A sarcoma tumor, which was induced in 1962 using 3-methylcholanthrene by Old et al. in Balb/c mice. |
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Organism | Mouse |
Tissue | Skin |
Disease | Fibrosarcoma |
Synonyms | Meth A, Meth-A, Meth-A-sarkom |
Characteristics
Age | Adult |
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Gender | Female |
Morphology | Round cells |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | Meth A sarcoma (Cytion catalog number 400284) |
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Biosafety level | 1 |
Expression / Mutation
Tumorigenic | Yes |
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Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Doubling time | 28 to 30 hours |
Subculturing | Allow cell aggregates to settle to the bottom of the flask, discard the supernatant medium, disperse the cells with gentle pipetting and dispense into new flasks. Resuspend cell suspension in the flask and take representative aliquot to count the cell number per ml. Dilute cell suspension to 1x10^5 cells/ml with fresh medium and transfer into new flasks. |
Split ratio | A ratio of 1:4 to 1:8 is recommended |
Seeding density | Start new cultures using 2 to 3 x 10^6 cells/ml. Once the cells have recovered from the freezing and thawing process after 1 to 2 passages, adjust the cell density to 1 x 10^6 cells/ml when splitting the cells. |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | About 53% of the initial cell number was collected after freezing. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | Amelogenin: x,y |