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> SARS-CoV-2 Papain-Like Protease Protein

SARS-CoV-2 Papain-Like Protease Protein

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Référence NB-64-53730-1mg

Conditionnement : 1mg

Marque : Neo Biotech

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RayPlex Human Anti-Viral Bead Array 1

This RayPlex® kit is a cytometric bead array to quantitatively measure, by flow cytometry, 14 human proteins which include interferons and interleukins. The assay has been optimized for serum, plasma, and cell culture supernatant.

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Product Description

Specifications

Size 192 tests, 48 tests, 480 tests, 96 tests
Species Human
Quantitative/Semi-Quantitative Quantitative
Number of Targets Detected 14
Compatible Sample Types Cell Culture Supernatants, Plasma, Serum
Solid Support Fluorescent Microbeads
Method Of Detection Flow Cytometry
Design Principle Sandwich-based
Estimated Lead Time 1-2 business days
Shipping Type Blue ice
Storage 4°C

Product Features

  • Less sample, more data: up to 25 proteins can be quantified from only 25 µl or less of your precious sample
  • 4 hour processing time
  • 5-10x more cost-effective than ELISA
  • Compatible with most flow cytometers
  • No specialized or dedicated instrument(s) needed
  • High specificity and system reproducibility
  • Well-suited for high throughput assays

Target Names

Scroll over each target protein for more information
GM-CSF
  • Gene Symbol: CSF2 GMCSF
  • Gene ID: 1437
  • Accession #:
  • ELISA Kit:
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 3458
  • Accession #:
  • ELISA Kit:
IL-1 beta
(IL-1 F2)
  • Gene Symbol: IL1B IL1F2
  • Gene ID: 3553
  • Accession #:
  • ELISA Kit:
IL-10
  • Gene Symbol: IL10
  • Gene ID: 3586
  • Accession #:
  • ELISA Kit:
IL-12 p70
  • Gene Symbol: IL12A NKSF1
  • Gene ID: 3592
  • Accession #:
  • ELISA Kit:
IL-15
  • Gene Symbol: IL15
  • Gene ID: 3600
  • Accession #:
  • ELISA Kit:
IL-18
  • Gene Symbol: IL18 IGIF IL1F4
  • Gene ID: 3606
  • Accession #:
  • ELISA Kit:
IL-2
  • Gene Symbol: IL2
  • Gene ID: 3558
  • Accession #:
  • ELISA Kit:
IL-6
  • Gene Symbol: IL6 IFNB2
  • Gene ID: 3569
  • Accession #:
  • ELISA Kit:
IL-8
(CXCL8)
  • Gene Symbol: CXCL8 IL8
  • Gene ID: 3576
  • Accession #:
  • ELISA Kit:
MIP-1 alpha
(CCL3)
  • Gene Symbol: CCL3 G0S19-1 MIP1A SCYA3
  • Gene ID: 6348
  • Accession #:
  • ELISA Kit:
MIP-1 beta
(CCL4)
  • Gene Symbol: CCL4 LAG1 MIP1B SCYA4
  • Gene ID: 388372 6351
  • Accession #:
  • ELISA Kit:
RANTES
(CCL5)
  • Gene Symbol: CCL5 D17S136E SCYA5
  • Gene ID: 6352
  • Accession #:
  • ELISA Kit:
TNF alpha
  • Gene Symbol: TNF TNFA TNFSF2
  • Gene ID: 7124
  • Accession #:
  • ELISA Kit:
 

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • RayPlex™ Multiplex Bead Cocktail
  • 1X Assay Diluent
  • 20X Wash Buffer
  • RayBio® Serum/Plasma Diluent
  • Lyophilized Protein Standard Mix
  • 1X Biotinylated Detection Antibody Cocktail
  • 10X Streptavidin-PE
  • Flow Cytometer Setup Bead Cocktail
  • V-bottom 96-well Microplate
  • Filter 96-well Microplate
Other Materials Required
  • Orbital 96-well plate shaker (with ability to reach 1000 rpm)
  • Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
  • Aluminum foil or 96-well Plate Aluminum Sealers
  • Distilled water
  • 1.5 ml polypropylene microcentrifuge tubes or similar
  • Microcentrifuge
  • Vacuum manifold compatible with a 96-well microplate if using the Filter 96-Well Microplate
  • Optional: 96-well round bottom plate (to prepare samples)
  • Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. !

Protocol Outline
  1. Prepare Reagents, Standards, and Samples
  2. Add RayPlex™ Multiplex Bead Cocktail to each well
  3. Incubate with Samples and Standards
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluorophore
  6. Analyze with a compatible flow cytometer
  7. Perform data analysis

Storage/Stability

Upon receipt, the Lyophilized Protein Standard Mix should be stored at -20°C or below. Other components should be stored at 4°C. Use reagents immediately after preparation. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.