Serotonin Antibody
Référence LS-C75755-100
Conditionnement : 100ul
Marque : LSBio
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Overview
Antibody:
Serotonin Goat Polyclonal Antibody
Application:
IHC, IHC-Pfa
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Specifications
Description
Serotonin antibody LS-C75755 is an unconjugated goat polyclonal antibody to Serotonin from human, mouse and rat. Validated for IHC. Cited in 1 publication.
(1) Publication(s)
Target
Serotonin
Host
Goat
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
Whole molecule 5HT.
Specificity
Recognizes mouse and rat Serotonin.
Applications
- IHC
- IHC - 4% paraformaldehyde (1:400 - 1:800)
Usage
Immunohistochemistry: 1:400-1:800. Fixation conditions for IHC: Perfusion Fixation: Fixative - 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4; 500ml, over approximately 20 minutes.Post Fixation: 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4; 500ml, over approximately 20 minutes.Post Fixation - 1.5 hour at 4°C. 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4.Note: Paraformaldehyde is a necessary component in fixation of serotonin. If needed, low levels of glutaraldehyde (0.1- 0.3%) may be used in conjunction with paraformaldehyde
Presentation
Lyophilized
Reconstitution
Reconstitute with 100ul of PBS or TBS
Storage
Store at -20°C lyophilized. Once reconstituted: Store at -20°C. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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