SK-LMS-1 growing culture
Référence 330125
Conditionnement : 1cellcultureflask
Marque : CLS Cell Lines Service
SK-LMS-1 Cells
General information
Organism | Human |
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Tissue | Vulvar |
Disease | Leiomyosarcoma |
Synonyms | SKLMS-1, SKLMS1 |
Characteristics
Age | 43 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Fibroblast-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | SK-LMS-1 (Cytion catalog number 300125) |
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Biosafety level | 1 |
Expression / Mutation
Antigen expression | Blood Type O, Rh+ |
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Isoenzymes | Me-2, 2, PGM3, 1-2, PGM1, 1-2, ES-D, 1, AK-1, 1, GLO-1, 1-2, G6PD, B, Phenotype Frequency Product: 0.0027 |
Tumorigenic | Yes, in nude mice. Forms leiomyosarcoma |
Karyotype | (P12) hypotriploid to hypertriploid (+A2, +A3, +C, +D, +E, +F, +G, -A) with abnormalities including dicentrics, acrocentric fragments, breaks, secondary constrictions, minutes and large submetacentric markers |
Handling
Culture Medium | DMEM:Ham's F12, w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:5 is recommended |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | Amelogenin: x,y CSF1PO: 9,1 D13S317: 12 D16S539: 8,11 D5S818: 11,13 D7S820: 8,9 TH01: 6,7 TPOX: 8,9 vWA: 18 D3S1358: 15,16 D21S11: 28,3 D18S51: 14,19 Penta E: 7,13 Penta D: 12,13 D8S1179: 12 FGA: 22,25 |