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SynaptoGreen™C4

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Référence 70020

Conditionnement : 5MG

Marque : Biotium

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SynaptoGreen™ C4 (Equivalent to FM®1-43)

A widely used green fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the red fluorescent dye SynaptoRed™ (70021), synapses or neuromuscular junctions can be imaged independently in two colors.

SynaptoGreen™ C4 (Equivalent to FM®1-43)

Product Attributes

CAS number

149838-22-2
Probe cellular localization

Membrane/vesicular
For live or fixed cells

For live/intact cells
Assay type/options

Real-time imaging
Colors

Green
Excitation/Emission

480/598 nm (in membranes)

Technology

Membrane Potential & Nerve Terminal Dyes
Neuronal Probes and Tools
Protocols
SDS
Supporting Documents

Product Description

A widely used green fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the red fluorescent dye SynaptoRed™ (70021), synapses or neuromuscular junctions can be imaged independently in two colors. Research results from the Tsien and Kay labs showed that brain slices can be stained with SynaptoGreen™ when SR101 (Sulforhodamine 101, 80101) or ADVASEP-7 (70029) is used to reduce the background staining. Biotium offers several Nerve Terminal Staining Kits for staining brain slices. Also see AM1-43 (70024), which is similar to SynaptoGreen™, but with a formaldehyde-fixable amine group.

SynaptoGreen™ also can be used to stain bacteria (gram-positive or gram-negative) and yeast, although in yeast it rapidly internalizes to vacuolar membranes. See our Cellular Stains Table for more information on how our dyes stain various organisms.

  • λEx/λEm: 510/625 nm (in MeOH); 480/598 nm (in membranes)
  • Red solid soluble in water
  • C30H49Br2N3
  • MW: 612


Nerve Terminal Dyes and Kits

Product Catalog No. Features
SynaptoGreen™ Dyes
(Ex/Em ~480/660 nm in membranes)		 70020... 70053 View full list of SynaptoGreen™ Dyes
SynaptoRed Dyes™
(Ex/Em ~510/750 nm in membranes)		 70021... 70050 View full list of SynaptoRed™ Dyes
Background Reducers
ADVASEP-7 70029 Sulfonated cyclodextrin that aids in removal of free dye during washes
SCAS 70037 Quenches extracellular fluorescences with fewer wash steps than ADVASEP-7
Sulforhodamine 101 80101 Red fluorescent dye that quenches extracellular fluorescence of SynaptoGreen™ dyes
Nerve Terminal Staining Kits
Nerve Terminal Staining Kit I 70030 Includes SynaptoGreen™ C4 and ADVASEP-7
Nerve Terminal Staining Kit II (A) 70031 Includes AM1-43 and ADVASEP-7
Nerve Terminal Staining Kit II (B) 70031-1 Includes AM1-43 and SCAS
Nerve Terminal Staining Kit III 70032 Includes SynaptoGreen™ C4 and Sulforhodamine 101
Nerve Terminal Staining Kit V 70034 Includes SynaptoRed™ C2 and ADVASEP-7
m is the number of carbons in the lipophilic tail and n is the number of double bonds linking the two aromatic rings in the dye.
**The positively-charged end of SynaptoRed C2M is a trimethylammonium group.
FM is a registered trademark of Thermo Fisher Scientific.
 

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