SynaptoRed™C2
Référence 70027
Conditionnement : 5x1MG
Marque : Biotium
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SynaptoRed™ C2 (Equivalent to FM®4-64)
A widely used red fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the green fluorescent dye SynaptoGreen™ (70020), synapses or neuromuscular junctions can be imaged independently in two colors. SynaptoRed™ has also been used to study endocytosis in Dictyostelia.
Technology
Membrane Potential & Nerve Terminal Dyes
Neuronal Probes and Tools
Protocols
Supporting Documents
Product Description
A widely used red fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the green fluorescent dye SynaptoGreen™ (70020), synapses or neuromuscular junctions can be imaged independently in two colors. SynaptoRed™ has also been used to study endocytosis in Dictyostelia. Please also see AM4-64 (70025), which is similar to SynaptoRed™, but with a formaldehyde-fixable amine group.
SynaptoRed™ can also be used to stain bacteria (gram-positive or gram-negative) and yeast, although in yeast it rapidly internalizes to vacuolar membranes. See our Cellular Stains Table for more information on how our dyes stain various organisms.
- λEx/λEm (membranes) = ~510/750 nm*
- Dark purple solid soluble in water
- C30H45Br2N3
- MW: 608
- [162112-35-8]
* Excitation/emission settings of 515/640 nm have been used for detection of yeast vacuole staining with SynaptoRed C2 (FM®4-64) (Ref. 4).
Nerve Terminal Dyes and Kits
| Product | Catalog No. | Features |
|---|---|---|
| SynaptoGreen™ Dyes (Ex/Em ~480/660 nm in membranes) | 70020... 70053 | View full list of SynaptoGreen™ Dyes |
| SynaptoRed Dyes™ (Ex/Em ~510/750 nm in membranes) | 70021... 70050 | View full list of SynaptoRed™ Dyes |
| Background Reducers | ||
| ADVASEP-7 | 70029 | Sulfonated cyclodextrin that aids in removal of free dye during washes |
| SCAS | 70037 | Quenches extracellular fluorescences with fewer wash steps than ADVASEP-7 |
| Sulforhodamine 101 | 80101 | Red fluorescent dye that quenches extracellular fluorescence of SynaptoGreen™ dyes |
| Nerve Terminal Staining Kits | ||
| Nerve Terminal Staining Kit I | 70030 | Includes SynaptoGreen™ C4 and ADVASEP-7 |
| Nerve Terminal Staining Kit II (A) | 70031 | Includes AM1-43 and ADVASEP-7 |
| Nerve Terminal Staining Kit II (B) | 70031-1 | Includes AM1-43 and SCAS |
| Nerve Terminal Staining Kit III | 70032 | Includes SynaptoGreen™ C4 and Sulforhodamine 101 |
| Nerve Terminal Staining Kit V | 70034 | Includes SynaptoRed™ C2 and ADVASEP-7 |
**The positively-charged end of SynaptoRed C2M is a trimethylammonium group.
FM is a registered trademark of Thermo Fisher Scientific.
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