Images
Western Blot (Tissue lysate)
Application

Western Blot (Tissue lysate)

Western Blot analysis of rat brain lysate (1) 1:100, (2) 1:250, (3) 1:500 and (4) 1:1000 of Syt7 monoclonal antibody.

Immunocytochemistry
Application

Immunocytochemistry

Immunocytochemical staining of SK-N-BE cell line. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Syt7 Antibody and (D) Composite.

  • Specifications

    Product Description

    Mouse monoclonal antibody raised against partial recombinant mouse Syt7.

    Immunogen

    Recombinant protein corresponding to amino acids 150-239 at cytoplasmic C2A domain of mouse Syt7.

    Host

    Mouse

    Reactivity

    Human, Mouse, Rat

    Specificity

    It does not cross react with synaptotagmin-6 or others. Can identify other isoforms bands at ~ 65kD.

    Form

    Liquid

    Conjugation

    ATTO 488

    Purification

    Protein G purification

    Isotype

    IgG2b

    Recommend Usage

    Immunocytochemistry (1:100)
    Immunofluorescence (1:100)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide).

    Storage Instruction

    Store at -20°C.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Tissue lysate)

    Western Blot analysis of rat brain lysate (1) 1:100, (2) 1:250, (3) 1:500 and (4) 1:1000 of Syt7 monoclonal antibody.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunocytochemistry

    Immunocytochemical staining of SK-N-BE cell line. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Syt7 Antibody and (D) Composite.

    Immunofluorescence

  • Gene Info — Syt7

    Entrez GeneID

    54525

    Protein Accession#

    Q9R0N7

    Gene Name

    Syt7

    Gene Alias

    AI851541, B230112P13Rik

    Gene Description

    synaptotagmin VII

    Gene Ontology

    Hyperlink

    Other Designations

    synaptotagmin 7