T-47D growing culture
Référence 330353
Conditionnement : 1cellcultureflask
Marque : CLS Cell Lines Service
T47D Cells
Information about T47D cells
Description | The T47D cell line, originating from the pleural effusion of an infiltrating ductal carcinoma of the breast, has become a critical resource in breast cancer research. T-47D cells are unique in the realm of cancer research for their hormonal expression profile, particularly for carrying receptors for 17 beta estradiol, various other steroids, and calcitonin. Additionally, T47D cells express the WNT7B oncogene. T47D cells are notable for their progesterone receptor expression not being regulated by estradiol, despite the hormone's abundance within the cells, setting them apart from MCF7 cells, which are widely recognized for their estrogen receptor positivity and are frequently used to explore estrogen's role in tumor proliferation and response to therapies. The utility of the T47D cell line extends to the formation of xenografts in immunodeficient mice, which are valuable for drug testing, observing receptor status changes, and studying angiogenesis. Furthermore, the T-47D cell line is a resource for cancer gene studies, providing insights into the genomic and proteomic landscape that drives breast cancer. By facilitating a deeper understanding of the proteomic and transcriptomic profiles of breast cancer, the t47d breast cancer cell line aids in the identification of new breast cancer cell phenotypes and the development of targeted therapies. T47D cells have been instrumental in studying the effects of hormones like progesterone on breast cancer, offering insights into transcriptional regulation, drug resistance, and the development of xenograft models for therapeutic testing. |
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Organism | Human |
Tissue | Breast |
Disease | Invasive ductal carcinoma |
Metastatic site | Pleural effusion |
Synonyms | T-47-D, T47-D, T47D:A, T47D |
Properties of the T47D cell line
Age | 54 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Monolayer, adherent |
Documentation
Citation | T-47D (Cytion catalog number 300353) |
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Biosafety level | 1 |
Genetic profile
Receptors expressed | Estradiol, steroids, calcitonin, androgen, progesterone, glucocorticoid, prolactin, estrogen |
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Isoenzymes | G6PD, B, PGM1, 1, PGM3, 1, ES-D, 2, Ak-1, 1, GLO-1, 1-2 |
Oncogenes | wnt3 +, wnt7h +, wnt7b+ |
Tumorigenic | Yes, in nude mice |
Mutational profile | TP53 mut |
Karyotype | Mode = 66, dicentric and extra long submetacentric chromosomes |
Handling T-47D breast cancer cells
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 to 1:5 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | Amelogenin: x,x CSF1PO: 11,13 D13S317: 12 D16S539: 10 D5S818: 12 D7S820: 11 TH01: 6 TPOX: 11 vWA: 14 D3S1358: 15,17 D21S11: 28,31 D18S51: 17 Penta E: 7,14 Penta D: 10,12 D8S1179: 13 FGA: 23 |
HLA alleles | A*: 33:01:01 B*: 14:02:01 C*: 08:02:01 DRB1*: 01:02:01 DQA1*: 01:01:02 DQB1*: 05:01:01 DPB1*: 02:01:02, 04:01:01 E: 01:01:01 |