TGFBI (NM_000358) Human Untagged Clone
Référence SC119927
Conditionnement : 10ug
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| Product Data | |
| Target Symbol | TGFBI |
|---|---|
| Synonyms | BIGH3; CDB1; CDG2; CDGG1; CSD; CSD1; CSD2; CSD3; EBMD; LCD1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | Fully Sequenced ORF >OriGene ORF within SC119927 sequence for NM_000358 edited (data generated by NextGen Sequencing) ATGGCGCTCTTCGTGCGGCTGCTGGCTCTCGCCCTGGCTCTGGCCCTGGGCCCCGCCGCG ACCCTGGCGGGTCCCGCCAAGTCGCCCTACCAGCTGGTGCTGCAGCACAGCAGGCTCCGG GGCCGCCAGCACGGCCCCAACGTGTGTGCTGTGCAGAAGGTTATTGGCACTAATAGGAAG TACTTCACCAACTGCAAGCAGTGGTACCAAAGGAAAATCTGTGGCAAATCAACAGTCATC AGCTACGAGTGCTGTCCTGGATATGAAAAGGTCCCTGGGGAGAAGGGCTGTCCAGCAGCC CTACCACTCTCAAACCTTTACGAGACCCTGGGAGTCGTTGGATCCACCACCACTCAGCTG TACACGGACCGCACGGAGAAGCTGAGGCCTGAGATGGAGGGGCCCGGCAGCTTCACCATC TTCGCCCCTAGCAACGAGGCCTGGGCCTCCTTGCCAGCTGAAGTGCTGGACTCCCTGGTC AGCAATGTCAACATTGAGCTGCTCAATGCCCTCCGCTACCATATGGTGGGCAGGCGAGTC CTGACTGATGAGCTGAAACACGGCATGACCCTCACCTCTATGTACCAGAATTCCAACATC CAGATCCACCACTATCCTAATGGGATTGTAACTGTGAACTGTGCCCGGCTCCTGAAAGCC GACCACCATGCAACCAACGGGGTGGTGCACCTCATCGATAAGGTCATCTCCACCATCACC AACAACATCCAGCAGATCATTGAGATCGAGGACACCTTTGAGACCCTTCGGGCTGCTGTG GCTGCATCAGGGCTCAACACGATGCTTGAAGGTAACGGCCAGTACACGCTTTTGGCCCCG ACCAATGAGGCCTTCGAGAAGATCCCTAGTGAGACTTTGAACCGTATCCTGGGCGACCCA GAAGCCCTGAGAGACCTGCTGAACAACCACATCTTGAAGTCAGCTATGTGTGCTGAAGCC ATCGTTGCGGGGCTGTCTGTAGAGACCCTGGAGGGCACGACACTGGAGGTGGGCTGCAGC GGGGACATGCTCACTATCAACGGGAAGGCGATCATCTCCAATAAAGACATCCTAGCCACC AACGGGGTGATCCACTACATTGATGAGCTACTCATCCCAGACTCAGCCAAGACACTATTT GAATTGGCTGCAGAGTCTGATGTGTCCACAGCCATTGACCTTTTCAGACAAGCCGGCCTC GGCAATCATCTCTCTGGAAGTGAGCGGTTGACCCTCCTGGCTCCCCTGAATTCTGTATTC AAAGATGGAACCCCTCCAATTGATGCCCATACAAGGAATTTGCTTCGGAACCACATAATT AAAGACCAGCTGGCCTCTAAGTATCTGTACCATGGACAGACCCTGGAAACTCTGGGCGGC AAAAAACTGAGAGTTTTTGTTTATCGTAATAGCCTCTGCATTGAGAACAGCTGCATCGCG GCCCACGACAAGAGGGGGAGGTACGGGACCCTGTTCACGATGGACCGGGTGCTGACCCCC CCAATGGGGACTGTCATGGATGTCCTGAAGGGAGACAATCGCTTTAGCATGCTGGTAGCT GCCATCCAGTCTGCAGGACTGACGGAGACCCTCAACCGGGAAGGAGTCTACACAGTCTTT GCTCCCACAAATGAAGCCTTCCGAGCCCTGCCACCAAGAGAACGGAGCAGACTCTTGGGA GATGCCAAGGAACTTGCCAACATCCTGAAATACCACATTGGTGATGAAATCCTGGTTAGC GGAGGCATCGGGGCCCTGGTGCGGCTAAAGTCTCTCCAAGGTGACAAGCTGGAAGTCAGC TTGAAAAACAATGTGGTGAGTGTCAACAAGGAGCCTGTTGCCGAGCCTGACATCATGGCC ACAAATGGCGTGGTCCATGTCATCACCAATGTTCTGCAGCCTCCAGCCAACAGACCTCAG GAAAGAGGGGATGAACTTGCAGACTCTGCGCTTGAGATCTTCAAACAAGCATCAGCGTTT TCCAGGGCTTCCCAGAGGTCTGTGCGACTAGCCCCTGTCTATCAAAAGTTATTAGAGAGG ATGAAGCATTAG Clone variation with respect to NM_000358.2 651 g=>c 5' Read Nucleotide Sequence >OriGene 5' read for NM_000358 unedited ATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAGCCGCCCGCT TGCCCGTCGGTCGCTAGCTCGCTCGGTGCGCGTCGTCCCGCTCCATGGCGCTCTTCGTGC GGCTGCTGGCTCTCGCCCTGGCTCTGGCCCTGGGCCCCGCCGCGACCCTGGCGGGTCCCG CCAAGTCGCCCTACCAGCTGGTGCTGCAGCACAGCAGGCTCCGGGGCCGCCAGCACGGCC CCAACGTGTGTGCTGTGCAGAAGGTTATTGGCACTAATAGGAAGTACTTCACCAACTGCA AGCAGTGGTACCAAAGGAAAATCTGTGGCAAATCAACAGTCATCAGCTACGAGTGCTGTC CTGGATATGAAAAGGTCCCTGGGNAGAAAGGGCTGTCCAGCAGCCCTACCACTCTCAAAC CTTTACGAGACCCTGGGAGTCGTTGGATCCACCACCACTCAGCTGTACACGGACCGCACG GAGAAGCTGAGGCCTGAGATGGAGGGGCCCGGCAGCTTCACCATCTTCGCCCCTAGCAAC GAGGCCTGGGCCTCCTTGCCAGCTGAAGTGCTGGACTCCCTGGTCAGCAATGTCAACATT GAGCTGCTCAATGCCCTCCGCTACCATATGGTGGGCAGGCGAGTCCTGACTGATGAGCTG NAACACGGCATGACCCTCACCTCTATGTACCAGAATTCCACATCCAGATCCACCACTATC CTAATGGGGATTGTAACCTGTGAACTGTGCCCGGCTCCTGAAAGCCGACCACCATGCACC AACGGGGGGTGGGGCACCTCAATCGATAAGGTCATTCTCCCCATCACCAACAACATCCAG CAGATAATTTGGGAACCGAGGACACCCTTGAGACCCTTCGGCCTGCTGTGGCTGGATCA 3' Read Nucleotide Sequence >OriGene 3' read for NM_000358 unedited GTCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTATGTTTCTTTGGTTT TATTATTACAAACACACCATGGCTCTGTCACAATAGGGAAATCTAAGCTCTACATACCAT TTCTTTTCTACCTGGACAGGGCCCCATAAAAGCCTCCAAGCCACGTGTAGATGTGAGACA CATTTGACAGAACATTTCAACTCATAGCTTATAATGATGCCATTTCTCCAGCTGTGCAAG GGCTTTACAAAAACTGTGCCAGGACTTCCCATGAGGCTGGATTGCTTGATTCATGTTTTA TGAGCCCCACAATACTGAAGCTCCTTTTCCAGGGACTTGGCATAGGCAGTCAATTCCACA TTTGGGATAGGTCCTCTCTGGAAGTGAATGTCAGGCAGTGACATCCAAGTTTCTGCATGC AGTGGGTTAACAGCCATGTTTAGGGGGAACATGATTTAAAAAGTACATCTCTCTCCCTCC TCCCCCACATGCACAAGGCTCACATCTCATTATGGTGCGGCCCATGTACATTAAAGTGTG ATACTTGGTTTTGAAACATTCAAACAGTCTCTGTGGAAATCTGACAGAAAATGGCGGAAA GCTGCCGCGGTGCATTCCTCCTGTAGTGCCTTACACCCAATGCTTCATCCTTTCTAATCC CTTCTGAAAACCGGGGGCCCTCCCCCAGACCTTTGGAAACCCCTGGAAAACGCTGATGCC TGGTCTCAGAACTCAACCCCATACTTGGAAGTTTATCCCCTTCTCCTGAGGTCCCGTGGT CGGTGGCTCCACACATTCGCCAGACCGGTCCCGCCCTCTTTGTGTCTCACGTCCATGTTA CCACCTCCCCCTATTACCCTACCCACTGCTTAAAACGCATTCCATCTGCACTCTTCCAAA TTTACCCCCACAGTGCCAGTTTTCCTCTCC |
| Restriction Sites | NotI-NotI Plasmid Map |
| ACCN | NM_000358 |
| Insert Size | 2800 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at info@clinisciences.com The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000358.1, NP_000349.1 |
| RefSeq Size | 2691 bp |
| RefSeq ORF | 2052 bp |
| Locus ID | 7045 |
| UniProt ID | Q15582 |
| Cytogenetics | 5q31.1 |
| Domains | FAS1 |
| Protein Families | Druggable Genome, Secreted Protein, Transmembrane |
| Summary | This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq, Jul 2008] |
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| SDS |
| SKU | Description | Size | ||
|---|---|---|---|---|
| RC200411 | TGFBI (Myc-DDK-tagged)-Human transforming growth factor, beta-induced, 68kDa (TGFBI) | 10 ug | | |
| RC200411L1 | Lenti ORF clone of Human transforming growth factor, beta-induced, 68kDa (TGFBI), Myc-DDK-tagged | 10 ug | | |
| RC200411L2 | Lenti ORF clone of Human transforming growth factor, beta-induced, 68kDa (TGFBI), mGFP tagged | 10 ug | | |
| RC200411L3 | Lenti ORF clone of Human transforming growth factor, beta-induced, 68kDa (TGFBI), Myc-DDK-tagged | 10 ug | | |
| RC200411L4 | Lenti ORF clone of Human transforming growth factor, beta-induced, 68kDa (TGFBI), mGFP tagged | 10 ug | | |
| RG200411 | TGFBI (tGFP-tagged) - Human transforming growth factor, beta-induced, 68kDa (TGFBI) | 10 ug | | |
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.