TNNT2 (Cardiac muscle Troponin T, Cardiomyopathy dilated 1D (autosomal dominant), Cardiomyopathy hypertrophic 2, CMD1D, CMH2, CMPD2, cTnT, LVNC6, MGC3889, OTTHUMP00000033864, OTTHUMP00000033865, OTTHUMP00000033866, OTTHUMP00000033867, OTTHUMP00000033870, OTTHUMP00000218095, RCM3, TNNT 2, TNNT2, TNNT2, TnTC, Troponin T Cardiac muscle, Troponin T Type 2 (Cardiac), Troponin T Type 2 Cardiac, Troponin T, Cardiac muscle, Troponin T2, Troponin T2 Cardiac)

Actin is a highly conserved protein that is expressed in all eukaryotic cells. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. Myosin is a hexamer of two heavy chains (MHC) and four light chains (MLC) that interacts with Actin to generate the force for diverse cellular movements, including cytokinesis, phagocytosis and muscle contraction. Troponin facilitates the interaction between Actin and Myosin by binding to calcium. Troponin is made up of at least two subunits, which are divergent in cardiac muscle, fast skeletal muscle and slow skeletal muscle. Structures of skeletal muscle troponin are composed of Troponin C (the sensor), Troponin I (the regulator) and Troponin T (the link to the muscle thin filament). Troponin C is dumbbell-shaped and has a hydrophobic pocket that increases the contractile force of muscle fibers. Troponin C has two isoforms: fast and slow. Fast Troponin C has two calcium binding sites, while slow/cardiac Troponin C has a single calcium binding site. ||Applications: |Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested.||Recommended Dilution:|Western Blot: 1:500-1:2000|Immunohistochemistry: 1:50-1:200 |Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.