Total Iron Binding Capacity (TIBC) Colorimetric Assay Kit
Detection principle
Performance characteristics
Synonyms | TIBC |
Sample type | Serum |
Sensitivity | 0.14 mg/L |
Detection range | 0.31-50 mg/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 20 min |
Precision | Average inter-assay CV: 2.300%Average intra-assay CV: 1.500% |
Other instruments required | Micropipettor, Water bath, Incubator, Vortex mixer, Centrifuge |
Storage | 2-8℃ |
Valid period | 12 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.31-50 mg/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type | Dilution factor |
Human serum | 1 |
Rat serum | 1 |
Porcine serum | 1 |
Rabbit serum | 1 |
Chicken serum | 1 |
Machin serum | 1 |
Note: The diluent is double distilled water.
Troubleshooting
Collection and treatment of common samples for metabolism assay detection
Questions and Answers of Metabolism Assay Kits
Citations
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Food & Function (2022) IF: 6.317
Arthrospira platensis (Spirulina) fortified functional foods ameliorates iron and protein malnutrition by improving growth, modulating oxidative stress and gut microbiota in ratsDOI: 10.1039/D2FO02226E
Sample: Serum