The kit is designed to prepare CUT&Tag libraries for mammalian cells for the Illumina high-throughput sequencing  platform. The prepared libraries can be used for Single-end sequencing and Paired-end sequencing. Cleavage Under  Target & Tagmentation (CUT&Tag) is a new method for studying protein interactions with DNA. Protein A/G-Tn5  transposase fusion protein and the target protein can form an immune complex through antibody bridging. Tn5  transposase is activated in the presence of Mg²⁺, and so short sequences containing adapters can be inserted into  both ends of the DNA fragment that binds to the target protein. Finally, library can be constructed by PCR  amplification. The kit is suitable for DNA research on the interaction of DNA-binding proteins such as transcription  factors and co-regulators. Compared with traditional ChIP-seq, it has the advantages of more accurate and stable  protein-DNA interaction information in low input of cells.

10-10⁵ mammalian cells, or processed cell nucleus, and eukaryotic cells without cell wall (such as plant cell protoplasts, etc.).

Box 1 is stored at 4°C for 1 year and Box 2 is stored at -20°C for 1 year

Box 1 ice bag (4℃) and Box 2 Dry ice (-70℃)