TRIL ELISA Kit (Rat)
Référence OKEH03645
Conditionnement : 96T
Marque : Aviva Systems Biology
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| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Rat, Rattus norvegicus | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology TRIL ELISA Kit (Rat) (OKEH03645) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tril has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Tril is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tril captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.156-10ng/ml | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 0.085 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Rat TLR4 interactor with leucine rich repeats | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | Tril |
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| Gene Full Name | TLR4 interactor with leucine-rich repeats |
| Alias Symbols | Leucine-rich repeat-containing protein KIAA0644 homolog;RGD1310827;TLR4 interactor with leucine rich repeats. |
| NCBI Gene Id | 362364 |
| Protein Name | Q496Z2 |
| Description of Target | TLR4 interactor with leucine rich repeats |
| Uniprot ID | https://www.uniprot.org/uniprot/Q496Z2 |
| Protein Accession # | NP_001029182.1 |
| Nucleotide Accession # | NM_001034010.1 |
| Protein Size (# AA) | 811 |
| Molecular Weight | 89 kDa |