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> α-Ketoglutarate (α-KG) Fluorometric Assay Kit

α-Ketoglutarate (α-KG) Fluorometric Assay Kit

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Katalog-Nummer E-BC-F047-96T

Size : 96T

Marke : Elabscience

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Telefonnummer : +1 850 650 7790

Detection Principle α-Ketoglutaric acid and alanine can be combined with fluorescent probe products under the action of a series of enzymes. The content of α-ketoglutaric acid in samples can be determined by measuring the fluorescence value.
Synonyms α-KG
Sample Type Serum,Plasma,Urine,Animal tissue,Cell sample
Detection Method Fluorometric method
Detection Instrument Fluorescence microplate reader (Ex/Em=535 nm/587 nm)
Research Area Tricarboxylic Acid（TCA）Cycle ,  Cuproptosis ,  Energy Metabolism
Other Reagents Required Normal saline (0.9% NaCl)
Storage This product can be stored at -20°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.60 μmol/L
Detection Range 0.60-100 μmol/L
Precision inter-assay CV: 0.04 | intra-assay CV: 0.02
Assay Time 35 min
Cat.No. Product Name Sizes
E-BC-K083-M α-Ketoglutarate Dehydrogenase (α-KGDH) Activity Assay Kit 96T
E-BC-K178-M Citrate Synthase (CS) Activity Assay Kit 96T , 48T
E-BC-K650-M Pyruvate Dehydrogenase (PDH) Activity Assay Kit 96T
E-BC-K651-M NAD-Isocitrate Dehydrogenase (NAD-IDH) Activity Assay Kit 96T
E-BC-K652-M Acetyl-CoA Colorimetric Assay Kit 96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
10% Rat kidney tissue homogenate 1
10% Rat lung tissue homogenate 1
10% Porcine heart tissue homogenate 1
10% Rat brain tissue homogenate 1
10% Mouse liver tissue homogenate 1
10^6 Jurkat cell 1
Human serum 1
Rat serum 2-3

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

Other Clones

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Other Formats

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Biotin

Chromogenic Biotin

Cyanine 3

Cyanine 5

Cyanine 5.5

Cyanine 7

Elab Fluor®405

Elab Fluor®430

Elab Fluor®488

Elab Fluor®568

Elab Fluor®594

Elab Fluor®647

Elab Fluor®680

Elab Fluor®700

Elab Fluor®750

Elab Fluor®Red 780

Elab Fluor®Violet 450

FITC

HRP

NHS-Biotin

NHS-LC-LC-Biotin

Sulfo-NHS-Biotin

Sulfo-NHS-LC-LC-Biotin

Unconjugated

detection instrument

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