10X PCR Buffer without MgCl2 Solution

Product Description

10X PCR Buffer without MgCl2
Catalog number: B2010121
Lot number: Batch Dependent
Expiration Date: Batch dependent
Volume/Weight: 4 x 1 mL
pH: 8.3
Supplied as: 10X concentrate (dilute to 1X with other PCR components before use)
Appearance: Clear solution
Applications: optimized buffer for routine PCR (dilute to 1X and add 1-4 mM MgCl2, 200 uM each dNTP).
Storage: −20°C
Keywords: PCR buffer without magnesium chloride
Grade: Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.

References:
1: Matsuda K. PCR-Based Detection Methods for Single-Nucleotide Polymorphism or
Mutation: Real-Time PCR and Its Substantial Contribution Toward Technological
Refinement. Adv Clin Chem. 2017;80:45-72.

2: Smith CJ, Osborn AM. Advantages and limitations of quantitative PCR
(Q-PCR)-based approaches in microbial ecology. FEMS Microbiol Ecol. 2009
Jan;67(1):6-20.

3: Romsos EL, Vallone PM. Rapid PCR of STR markers: Applications to human
identification. Forensic Sci Int Genet. 2015 Sep;18:90-9.

4: Krawczyk B, Kur J, Stojowska-Swędrzyńska K, Śpibida M. Principles and
applications of Ligation Mediated PCR methods for DNA-based typing of microbial
organisms. Acta Biochim Pol. 2016;63(1):39-52.

5: Green MR, Sambrook J. Analysis and Normalization of Real-Time Polymerase
Chain Reaction (PCR) Experimental Data. Cold Spring Harb Protoc. 2018 Oct
1;2018(10).

6: Hapuarachchi CT, Jeffery KJM, Bowler ICJW. Stool PCR may not be a substitute
for enrichment culture for the detection of salmonella. J Med Microbiol. 2019
Mar;68(3):395-397.

7: Ehnert S, Linnemann C, Braun B, Botsch J, Leibiger K, Hemmann P, Nussler AAK.
One-Step ARMS-PCR for the Detection of SNPs-Using the Example of the
PADI4 Gene. Methods Protoc. 2019 Jul 25;2(3):63.

8: Dahiya B, Mehta PK. Detection of potential biomarkers associated with
outrageous diseases and environmental pollutants by nanoparticle-based immuno-
PCR assays. Anal Biochem. 2019 Dec 15;587:113444.

9: Doyle L, Vogel S, Procop GW. Pneumocystis PCR: It Is Time to Make PCR
the Test of Choice. Open Forum Infect Dis. 2017 Sep 2;4(4):ofx193.

10: Amoupour M, Nezamzadeh F, Bialvaei AZ, Sedighi M, Jazi FM, Alikhani MY,
Mirnejad R. Differentiation of Brucella abortus and B. melitensis
biovars using PCR-RFLP and REP-PCR. New Microbes New Infect. 2019 Aug
22;32:100589.

Products Related to 10X PCR Buffer without MgCl2 Biochemical Buffers and Solutions