Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie

Acid Phosphatase (ACP) Activity Assay Kit

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Katalog-Nummer E-BC-K094-M-500

Size : 500Assays

Contact local distributor :

Telefonnummer : +1 850 650 7790

Marke : Elabscience

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Contact local distributor :

Telefonnummer : +1 850 650 7790

Detection Principle	Acid phosphatase decomposes disodium phenyl phosphate under acidic conditions to produce free phenol and phosphoric acid. Phenol acts with 4-aminoantipyrine in alkaline solution, and oxidizes to a derivative of red quinone by potassium ferricyanide. The activity of the ACP can be calculated by measuring the OD value at 520 nm.
Synonyms	ACP
Sample Type	Serum,Plasma,Hydrothorax,Urine,Cell,Cell culture supernatant,Animal tissue
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (510-530 nm, optimum wavelength: 520 nm)
Research Area	Amino Acids And Proteins
Other Reagents Required	Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	1.40 U/100 mL
Detection Range	1.40-40 U/100 mL
Precision	inter-assay CV: 9.4% \| intra-assay CV: 2.1%
Assay Time	45 min

Cat.No.	Product Name	Sizes
E-BC-K091-M	Alkaline Phosphatase (ALP) Activity Assay Kit	500Assays , 96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human serum	1
Humasn saliva	1
Chicken serum	1
Human urine	1
10% Rat liver tissue homogenate	4-10
10% Rat kidney tissue homogenate	4-10
HepG2 cells	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.