Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie

Adenosine Deaminase (ADA) Activity Assay Kit

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Katalog-Nummer E-BC-K197-M-96T

Size : 96T

Contact local distributor :

Telefonnummer : +1 850 650 7790

Marke : Elabscience

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Contact local distributor :

Telefonnummer : +1 850 650 7790

Detection Principle	Adenosine deaminase (ADA) can hydrolyzed the substrate adenosine to form hypoxanthine riboside, which is hydrolyzed by purine riboside phosphatase to produce hypoxanthine and phosphate ribose. Under the action of xanthine oxidase, hypoxanthine produces hydrogen peroxide, which produces red substance under the action of peroxidase, 4-aminotepyrine and color source. The red substance has the maximum absorption peak at 550 nm and the changes of absorbance is proportional to the activity of ADA.
Synonyms	ADA
Sample Type	Serum,Plasma,Animal tissue
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (550 nm)
Research Area	Liver And Renal Function
Other Reagents Required	Normal saline (0.9% NaCl)
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.03 U/L
Detection Range	0.03-99 U/L
Precision	inter-assay CV: 0.06 \| intra-assay CV: 0.03
Assay Time	40 min

Cat.No.	Product Name	Sizes
E-BC-K235-M	Alanine Aminotransferase (ALT/GPT) Activity Assay Kit	96T , 48T
E-BC-K236-M	Aspartate Aminotransferase (AST/GOT) Activity Assay Kit	96T , 48T
E-BC-K760-M	Total Bilirubin (TBIL) Colorimetric Assay Kit	96T , 48T
E-BC-K761-M	Direct Bilirubin (DBIL) Colorimetric Assay Kit	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Mouse liver tissue homogenate	1
10% Rat kidney tissue homogenate	1
10% Rat heart tissue homogenate	2-3
10% Rat spleen tissue homogenate	2-3
Mouse serum	1
Human serum	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.