ADH1 Rabbit Polyclonal Antibody
Katalog-Nummer R1049BS
Size : 100ug
Product Data | |
Application | ELISA, IP, WB |
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Application | Western blot: 1/500-1/5,000. Immunoprecipitation: 1/100. ELISA: 1/5,000-1/20,000. Antibody has been assayed against 1.0 ug of Alcohol dehydrogenase in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested. |
Reactivity | Yeast |
Antibody Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Alcohol dehydrogenase from yeast |
Specificity | This antibody detects yeast alcohol dehydrogenase. Cross reactivity against alcohol dehydrogenase from other sources may occur but have not been specifically determined. Immunoelectrophoresis gives a single precipitin arc against anti-Biotin, anti-rabbit serum as well as purified and partially purified yeast alcohol dehydrogenase. |
Buffer | 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: Biotin State: Lyophilized purified Ig faction Stabilizer: 10 mg/m BSA (immunoglobulin and protease free) Preservative: 0.01% Sodium azide |
Reconstitution Method | Restore with 0.1 ml of deionized water (or equivalent). |
Concentration | lot specific |
Purification | Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
Conjugation | Biotin |
Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Database Link | |
Synonyms | Alcohol dehydrogenase I, YADH-1, ADH1, ADC1, YOL086C, O0947 |
Reference Data |
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