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> Aflatoxin B1 [1162-65-8]

Aflatoxin B1 [1162-65-8]

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Katalog-Nummer HY-N6615-1mg

Size : 1mg

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Aflatoxin B1 (AFB1) is a Class 1A carcinogen, which is a secondary metabolite of Aspergillus flavus and A. parasiticus. Aflatoxin B1 (AFB1) mainly induces the transversion of G-->T in the third position of codon 249 of the p53 tumor suppressor gene, resulting in mutation.

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Aflatoxin B1 Chemische Struktur

Aflatoxin B1 Chemische Struktur

CAS. Nr. : 1162-65-8

This product is a controlled substance and not for sale in your territory.

Based on 3 publication(s) in Google Scholar

Other Forms of Aflatoxin B1:

  • Aflatoxin B1 (Standard) Angebot einholen
  • Aflatoxin B1-13C17 Angebot einholen

Alle Parasite Isoform-spezifische Produkte anzeigen:

Alle Isoformen anzeigen

Beschreibung

Aflatoxin B1 (AFB1) is a Class 1A carcinogen, which is a secondary metabolite of Aspergillus flavus and A. parasiticus. Aflatoxin B1 (AFB1) mainly induces the transversion of G-->T in the third position of codon 249 of the p53 tumor suppressor gene, resulting in mutation[1][2].

IC50 & Target

Microbial Metabolite

 

Molekulargewicht

312.27

Formel

C17H12O6
CAS. Nr.

1162-65-8
Appearance

Solid

Color

White to yellow

SMILES

COC1=CC(O[C@]2(19)[C@@]3(19)C=CO2)=C3C(O4)=C1C(CCC5=O)=C5C4=O
Initial Source
  • Endogenous metabolite
  • Human Gut Microbiota Metabolites
  • Microorganisms

Aspergillus flavus;A. parasiticus
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, protect from light

*In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)

Lösungsmittel & Löslichkeit
In Vitro: 

DMF : 33.33 mg/mL (106.73 mM; Need ultrasonic)

DMSO : 30 mg/mL (96.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2024 mL 16.0118 mL 32.0236 mL
5 mM 0.6405 mL 3.2024 mL 6.4047 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

  • Molaritätsrechner

  • Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 3 mg/mL (9.61 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 3 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation

Purity: 99.96%

COA (252 KB) HNMR (252 KB) LCMS (93 KB)

Handling Instructions (2659 KB)
Verweise

  • [1]. Gizachew D, et al. Aflatoxin B1 (AFB1) production by Aspergillus flavus and Aspergillus parasiticus on ground Nyjer seeds: The effect of water activity and temperature. Int J Food Microbiol. 2019 May 2;296:8-13.  [Content Brief]

    [2]. Aguilar F, et al. Aflatoxin B1 induces the transversion of G-->T in codon 249 of the p53 tumor suppressor gene in human hepatocytes. Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8586-90.  [Content Brief]

  • [1]. Gizachew D, et al. Aflatoxin B1 (AFB1) production by Aspergillus flavus and Aspergillus parasiticus on ground Nyjer seeds: The effect of water activity and temperature. Int J Food Microbiol. 2019 May 2;296:8-13.

    [2]. Aguilar F, et al. Aflatoxin B1 induces the transversion of G-->T in codon 249 of the p53 tumor suppressor gene in human hepatocytes. Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8586-90.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / DMF 1 mM 3.2024 mL 16.0118 mL 32.0236 mL 80.0589 mL
5 mM 0.6405 mL 3.2024 mL 6.4047 mL 16.0118 mL
10 mM 0.3202 mL 1.6012 mL 3.2024 mL 8.0059 mL
15 mM 0.2135 mL 1.0675 mL 2.1349 mL 5.3373 mL
20 mM 0.1601 mL 0.8006 mL 1.6012 mL 4.0029 mL
25 mM 0.1281 mL 0.6405 mL 1.2809 mL 3.2024 mL
30 mM 0.1067 mL 0.5337 mL 1.0675 mL 2.6686 mL
40 mM 0.0801 mL 0.4003 mL 0.8006 mL 2.0015 mL
50 mM 0.0640 mL 0.3202 mL 0.6405 mL 1.6012 mL
60 mM 0.0534 mL 0.2669 mL 0.5337 mL 1.3343 mL
80 mM 0.0400 mL 0.2001 mL 0.4003 mL 1.0007 mL
DMF 100 mM 0.0320 mL 0.1601 mL 0.3202 mL 0.8006 mL
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Aflatoxin B1 Related Classifications

  • Induced Disease Models Products
  • Cancer Models Immunology and Inflammatory Disease Models
  • Liver Cancer Models Hapten
  • Anti-infection Metabolic Enzyme/Protease
  • Bacterial Endogenous Metabolite Parasite
Help & FAQs

Keywords:

Aflatoxin B11162-65-8Aflatoxin B 1Aflatoxin B-1BacterialEndogenous MetaboliteParasiteInhibitorinhibitorinhibit

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