Alanine Transaminase (ALT) Assay Kit is an assay kit designed for the detection and quantification of Alanine Transaminase (ALT) activity in serum, plasma, tissue homogenates, cell lysates and cell culture supernatants using a microplate reader. ALT catalyzes the amino conversion reaction between alanine and alpha-ketoglutaric acid to produce pyruvic acid and glutamic acid. Phenylhydrazine can be added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish-brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.
Research Articles on Alanine Transaminase (ALT)
| Target | Alanine Transaminase (ALT) |
| Reactivity | General (All species) |
| Storage | Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual. |
| Validity | The validity for this kit is 12 months. |
| Test Range | 0.75 IU/L - 72.3 IU/L |
| Sensitivity | 0.75 IU/L |
| Detection Method | Colorimetric |
| Assay Data | Quantitative |
| Sample Type | Serum, plasma, tissue homogenates, cell lysates and cell culture supernatants. |
| CAS Number | 9000-86-6 |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |