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> AMBP Polyclonal Antibody

AMBP Polyclonal Antibody

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Katalog-Nummer E-AB-40503-200

Size : 200uL

Marke : Elabscience

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Verified Samples Verified Samples in WB: Zebrafish Zebrafish, Human plasma, Human serum
Dilution WB 1:1000-2000
Isotype IgG
Host Rabbit
Reactivity Human,  Zebrafish
Applications WB
Clonality Polyclonal
Immunogen Recombinant human AMBP protein expressed by E.coli
Abbre AMBP
Synonyms AMBP,   Alpha 1 microglobulin/bikunin precursor,   Alpha 1 microglycoprotein,   Alpha-1 microglycoprotein,   Alpha-1-microglobulin,   Bikunin,   Complex-forming glycoprotein heterogeneous in charge,   EDC1,   Growth inhibiting protein 19,   HCP,   HI-30,   HI30,   IAT,  A1M
Swissprot
Calculated MW 39 kDa
Observed MW 39 kDa

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization Secreted
Concentration 1 mg/mL
Buffer PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method Antigen Affinity Purification
Research Areas Cancer,  Cell Biology,  Cardiovascular
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background AMBP, alpha-1-microglobulin/bikunin precursor, has been reported to interact with albumin,The plasma concentration of albumin-AMBP was estimated to be 5.2 mg/L．alpha 1 Microglobulin runs at approximately 27 kDa under reducing conditions and as a dimer at
Cat.No. Product Name Sizes
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E-AB-1043 Streptavidin(peroxidase/HRP conjugated) 500μL , 120μL , 60μL
E-AB-1194 HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed 500μL , 120μL , 1mL
E-IR-R304A Western Blot Detection Kit 50Assays
E-IR-R304B High Accuracy and Absorbability Western Blot Detection Kit 50Assays
Other Clones

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Other Formats

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Unconjugated

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