Androgen receptor Polyclonal Antibody
Katalog-Nummer bs-0118R-20ul
Size : 20ul
Marke : Bioss
Contact local distributor :
Telefonnummer : +1 850 650 7790
Androgen receptor Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Androgen Receptor Polyclonal Antibody, Unconjugated (bs-0118R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0118r-100ul |
| Product Name | Androgen receptor Polyclonal Antibody |
| Applications | IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC) |
| Reactivity | Human, Mouse, Rat, Pig, Goat |
| Predicted Reactivity | Cow, Sheep, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human AR |
| Immunogen Range | 825-919/919 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 367 |
| Swiss Prot | P10275 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | KD; AIS; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA; Androgen receptor; Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4; AR |
| Background | Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3. |
| Application Dilution | |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |