Application
| WB, IHC, ICC |
---|---|
Primary Accession | P16157 |
Other Accession | NP_000028.3 |
Host | Mouse |
Isotype | IgG1 |
Reactivity | Human, Mouse, Rat |
Clonality | Monoclonal |
Description | Mouse Anti-Human Ankyrin (pan) Monoclonal IgG1 |
Target/Specificity | Detects ~200kDa. Cross-reacts with Ankyrin-B and Ankyrin-G. |
Other Names | Ankyrin-1 Antibody, Erythrocyte Ankyrin Antibody, ANK-1 Antibody, ANK Antibody, rCG_43073 Antibody |
Clone Names | S388A-60 |
Immunogen | Fusion protein amino acids 1-1881 (full-length) of human Ankyrin-R |
Purification | Protein G Purified |
Storage | -20ºC |
Storage Buffer | PBS pH7.4, 50% glycerol, 0.1% sodium azide |
Shipping Temperature | Blue Ice or 4ºC |
Certificate of Analysis | A 1:100 dilution of SMC-488 was sufficient for detection of Ankyrin (pan) in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Cellular Localization | Cytoplasm | Cytoskeleton | Myofibril | Sarcomere | M line |
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Provided below are standard protocols that you may find useful for product applications.
Background
Ankyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton (1). Ankyrins have binding sites for the beta subunit of spectrin and at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers and ion transporters in the plasma membrane.
References
1. Bennett V., Baines A.J. (2001) Physiol. Rev. 81 (3): 1353–92.