31-1022-00 | Anti-Human IgG4 rabbit monoclonal antibody [RM120] Clinisciences

Product Details

Product Name Anti-Human IgG4 Rabbit Monoclonal Antibody, Clone RM120

Product Description Rabbit monoclonal to Human IgG4

Catalog No. 31-1022-00

Clone Name RM120

Product Data Sheet Anti-Human IgG4 Rabbit Monoclonal Antibody Data Sheet RM120

Specificity RM120 reacts to the heavy chain of human IgG4, RM120 does not cross react to any other IgG subclasses (IgG1, IgG2, or IgG3), and shows no cross reactivity to IgM, IgA, IgD, IgE. RM120 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.

Immunogen Peptide corresponding to the hinge region of Human IgG4

Application ELISA
Western Blot
Immunohistochemistry (IHC)
Immunocytochemistry (ICC)
Flow Cytometry (FC)

Species Reactivity Human

Conjugate None

Intended Use Research Use Only

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Properties

Form Liquid

Storage Instructions Store at -20.0°C

Stability Stable for 1 Year at -20.0°C from date of receipt

Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Volume/Item Size 100 µg

Concentration 1.0 mg/mL

Purity Protein A affinity purified from an animal origin–free culture supernatant

Clone Type Monoclonal

Isotype Rabbit IgG

Usage ELISA: 50 ng/well – 200 ng/well (for Capture);
0.05 µg/mL – 0.2 µg/mL (for Detection);
Western Blot (WB): 0.5 µg/mL – 2 µg/mL;
Immunocytochemistry (ICC): 1 µg/mL - 10 µg/mL;
Immunohistochemistry (IHC): 1 µg/mL - 10 µg/mL;
Flow Cytometry (FC): 1 µg/mL - 4 µg/mL.

References for Human IgG4 antibody [RM120] Sulka KB et al. Lyme Disease-Associated IgG4 Autoantibodies Correlate with Synovial Pathology in Antibiotic-Refractory Lyme Arthritis. Arthritis Rheumatol. (2018) 10.1002/art.40566. IHC-P; Human. Read more (PubMed: 29790305)

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Product Images

Detection
Detection of human IgG4 in monkey serum. Sandwich ELISA using RM120 as the capture antibody, and biotinylated anti-human light chains (κ+ λ) antibody RM129 as the detection antibody, followed by a HRP conjugated streptavidin.

Sandwich ELISA using RM120 as the capture antibody, and biotinylated anti-human light chains (κ+ λ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin.

Western
Western Blot of human, mouse, rat, and goat IgG shows RM120 reacts to human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms. No cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG.

A titer ELISA using RM120. The plate was coated with different amounts of human IgG4. A serial dilution of RM120 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Immunohistochemistry
Immunohistochemical staining of formalin fixed and paraffin embedded human lymphoid tissue section using anti-human IgG4 rabbit monoclonal antibody, Clone RM120.

ELISA
ELISA showing RM120 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM120 and a monkey IgG binding antibody (positive control) was used as the detection antibody.

Immunohistochemical staining of FFPE Tonsil tissue sections using Anti-Human IgG4 antibody, Clone RM120, at a 1:50,000 dilution.

Image courtesy of Shaomin Hu, MD, PhD, Department of Anatomic and Clinical Pathology. Montefiore Medical Center Albert Einstein College of Medicine. Bronx, New York, USA

Anti-Human IgG4 rabbit monoclonal antibody [RM120]

Katalog-Nummer 31-1022-00

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Marke : RevMab Biosciences

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