General Info
| Host: | Mouse |
| Applications: | FC/WB/IF/IHC |
| Reactivity: | Cow/Dog/Ferret/Hamster/Human/Mouse/Pig/Rat/Sheep |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Mouse monoclonal antibody anti-Keratin 5+8 is suitable for use in Flow Cytometry, Western Blot, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality: | Monoclonal |
| Clone ID: | C-50 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1k |
| Formulation: | PBS with 0.02% Sodium Azide. |
| Purification: | Affinity purified from tissue culture. |
| Concentration: | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Dilution Range: | Flow Cytometry (1-2 µg/million cells in 0.1 ml, fix cells in 4% PFA for 10 min, at 4°C, permeabilize with 0, 2% saponin or digitonin for 15 min, at 4°C). Immunoblotting (0.5-1 µg/ml). Immunofluorescence (1-2 µg/ml). Immunohistology (1-2 µg/ml for 30 |
| Storage Instruction: | Store for up to 1 year at 2-8°C upon receipt. |
Information
| Immunogen: | A BALB/c mouse was immunized with a cytoskeleton preparation of HeLa cells. Fusion partner: Sp2/0. |
Description
| Background | Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance