75-021 | Anti-Kvbeta2 K+ Channel Antibody Clinisciences

Anti-Kvbeta2 K+ Channel Antibody (K17/70)

Our Anti-Kvbeta2 K+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone K17/70. It is KO validated, detects finch, human, mouse, non-human primate, rat, and Xenopus Kvbeta2 K+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Immunoblot on adult mouse brain membranes from two wild-type (WT1,2) and Kvβ2 knockout (Kvβ2 KO) mice. Brain samples courtesy of Dr. Geoff Murphy, Molecular and Behavioral Neuroscience Institute, University of Michigan.

Adult rat hippocampus immunohistochemistry.

Adult rat brain membrane immunoblot.

Species Reactivity

Finch, Human, Mouse, Non-Human Primate, Rat, Xenopus

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Data Sheet

KO Validated

SKU: 75-021

Product Details

Target

Kvbeta2 K+ channel

Target Description

Voltage-gated potassium channel subunit beta-2 or Kvß2 is encoded by the gene KCNAB2. Kvß2 is a member of the potassium channel, voltage-gated, shaker-related subfamily. Kvβ2 is a cytoplasmic subunit that modulates the characteristics of the membrane spanning, channel-forming alpha-subunits. Beta subunits can promote the closure of channels or enhance channel activity depending on other channel proteins. Kvβ2 is expressed in brain, in thecerebellum and in the juxtaparanodal region of nodes of Ranvier. Kvβ2 expression is also detected in lung, heart and kidney. Diseases associated with KCNAB2 include Chromosome 1P36 Deletion Syndrome and Juvenile Amyotrophic Lateral Sclerosis 16,

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

K17/70

Isotype

IgG1

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Kcnab2 Ckbeta2 I2rf5 Kcnb3

Molecular Weight

38 kDa (major band), 41 kDa (minor band)

Antigen

Fusion protein amino acids 1-367 (full length) of rat Kv2 (accession number NP_034728) produced recombinantly in E. Coli

Species Reactivity

Finch, Human, Mouse, Non-Human Primate, Rat, Xenopus

Antibody Registry ID

AB_2131373

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:500
IHC: 1:250
ICC: 1:100

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Voltage-gated potassium channel subunit beta-2 (EC 1.1.1.-) (K(+) channel subunit beta-2) (Kv-beta-2) (Neuroimmune protein F5)

UniProt Details

UniProt (Human): Q13303
UniProt (Immunogen Species): P62482

Product Specific References for Applications and Species

Immunocytochemistry: Mouse | Rat
Immunohistochemistry: Mouse | Rat | Zebra Finch
Immunoprecipitation: Human | Mouse | Rat
PLA: Mouse
Western Blot: Human | Mouse | Non-Human Primate | Rat | Xenopus

Immunocytochemistry: Mouse
PMID	Dilution	Publication
18988673	1:100	Ernst, S.J., et al. 2009. Sodium channel beta1 regulatory subunit deficiency reduces pancreatic islet glucose-stimulated insulin and glucagon secretion.. Endocrinology, 1132-9.
17651419	not listed	Wenzel, H. J., et al. 2007. Structural consequences of Kcna1 gene deletion and transfer in the mouse hippocampus.. Epilepsia, 2023-46.

Immunocytochemistry: Rat
PMID	Dilution	Publication
23318870	not listed	Ovsepian, S. V., et al. 2013. A defined heteromeric KV1 channel stabilizes the intrinsic pacemaking and regulates the output of deep cerebellar nuclear neurons to thalamic targets.. The Journal of Physiology, 1771-91.
21602278	not listed	Gu, C., et al. 2011. Clustering and activity tuning of Kv1 channels in myelinated hippocampal axons.. The Journal of Biological Chemistry, 25835-47.
18509034	not listed	Ogawa, Y., et al. 2008. Postsynaptic density-93 clusters Kv1 channels at axon initial segments independently of Caspr2.. Journal of Neuroscience, 5731-9.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
26269648	not listed	Kole, M.J., et al. 2015. Selective loss of presynaptic potassium channel clusters at the cerebellar basket cell terminal pinceau in adam11 mutants reveals their role in ephaptic control of purkinje cell firing. . Journal of Neuroscience, 11433-11444.
22560931	not listed	Jukkola, P. I., et al. 2012. K+ channel alterations in the progression of experimental autoimmune encephalomyelitis.. Neurobiology of Disease, 280-93.
21958379	not listed	Duflocq, A., et al. 2011. Characterization of the axon initial segment (AIS) of motor neurons and identification of a para-AIS and a juxtapara-AIS, organized by protein 4.1B.. BMC Biology, 6.
20197544	1:250	Stapels, M., et al. 2010. Polycomb group proteins as epigenetic mediators of neuroprotection in ischemic tolerance.. Science Signaling, ra15.

Immunohistochemistry: Rat
PMID	Dilution	Publication
22764231	not listed	Williams, M. R., et al. 2012. Cellular mechanisms and behavioral consequences of Kv1.2 regulation in the rat cerebellum.. Journal of Neuroscience, 9228-37.
18727953	not listed	Monaghan, M. M., et al. 2008. Altered expression and localization of hippocampal A-type potassium channel subunits in the pilocarpine-induced model of temporal lobe epilepsy.. Neuroscience, 550-562.

Immunohistochemistry: Zebra Finch
PMID	Dilution	Publication
23818387	1:250	Condro, M. C., et al. 2014. Distribution of language-related Cntnap2 protein in neural circuits critical for vocal learning.. Journal of Comparative Neurology, 169-85.

PLA: Mouse
PMID	Dilution	Publication
31639389	not listed	Kilfoil, P.J., et al. 2019. Metabolic regulation of Kv channels and cardiac repolarization by Kvβ2 subunits.. Journal of Molecular and Cellular Cardiology, 93-106.

Western Blot: Human
PMID	Dilution	Publication
35396942	1:1000	Roig, S.R., et al. 2022. S-acylation-dependent membrane microdomain localization of the regulatory Kvβ2. 1 subunit. . Cellular and Molecular Life Sciences, 230.

Western Blot: Mouse
PMID	Dilution	Publication
35440632	1:400	Dwenger, M.M., et al. 2022. Pyridine Nucleotide Redox Potential in Coronary Smooth Muscle Couples Myocardial Blood Flow to Cardiac Metabolism. Nature Communications, 2051.
31639389	10 µg	Kilfoil, P.J., et al. 2019. Metabolic regulation of Kv channels and cardiac repolarization by Kvβ2 subunits.. Journal of Molecular and Cellular Cardiology, 93-106.
31639389	1:100	Kilfoil, P.J., et al. 2019. Metabolic regulation of Kv channels and cardiac repolarization by Kvβ2 subunits.. Journal of Molecular and Cellular Cardiology, 93-106.
31639389	1:100	Kilfoil, P.J., et al. 2019. Metabolic regulation of Kv channels and cardiac repolarization by Kvβ2 subunits. Journal of molecular and cell cardiology, 93–106.
29476105	1:1000	Nguyen, L.H., et al. 2018. mTOR-dependent alterations of Kv1.1 subunit expression in the neuronal subset-specific Pten knockout mouse model of cortical dysplasia with epilepsy. Scientific Reports, 3568.
26269648	1:200	Kole, M.J., et al. 2015. Selective loss of presynaptic potassium channel clusters at the cerebellar basket cell terminal pinceau in adam11 mutants reveals their role in ephaptic control of purkinje cell firing. . Journal of Neuroscience, 11433-11444.
24036102	not listed	Bavassano, C., et al. 2013. Identification of voltage-gated K(+) channel beta 2 (Kvβ2) subunit as a novel interaction partner of the pain transducer Transient Receptor Potential Vanilloid 1 channel (TRPV1).. Biochimica et Biophysica Acta, 3166-75.
21507952	not listed	Cheng, L., et al. 2011. Cortactin is required for N-cadherin regulation of Kv1.5 channel function.. The Journal of Biological Chemistry, 20478-20489.
20685990	not listed	Ohnishi, H., et al. 2010. Stress-evoked tyrosine phosphorylation of signal regulatory protein α regulates behavioral immobility in the forced swim test.. Journal of Neuroscience, 10472-10483.

Western Blot: Non-Human Primate
PMID	Dilution	Publication
22426702	not listed	Tipparaju, S. M., et al. 2012. Interactions between the C-terminus of Kv1.5 and Kvβ regulate pyridine nucleotide-dependent changes in channel gating.. Pflügers Archiv: European Journal of Physiology, 799-818.

Western Blot: Rat
PMID	Dilution	Publication
17145502	not listed	Gu, C., et al. 2006. The microtubule plus-end tracking protein EB1 is required for Kv1 voltage-gated K+ channel axonal targeting.. Neuron, 803-16.

Western Blot: Xenopus
PMID	Dilution	Publication
18684900	1:50	Pineda, R.H, et al. 2008. Kv1 potassium channel complexes in vivo require Kvβ2 subunits in dorsal spinal neurons. . Journal of neurophysiology, 2125-2136.

Anti-Kvbeta2 K+ Channel Antibody

Katalog-Nummer 75-021

Contact local distributor :

Marke : Antibodies Incorporated

Contact local distributor :

Anti-Kvbeta2 K+ Channel Antibody (K17/70)

SKU: 75-021

Product Details

Product Specific References for Applications and Species

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