Anti-OsTIR1 pAb
Katalog-Nummer PD048
Size : 100ul
Marke : MBL International
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Telefonnummer : +1 850 650 7790
Please Note: MBL International will be shutting down its operations effective December 31, 2024. Distribution of MBL products in the United States will be transferred to Cosmo Bio US on January 1st while European Distributors will remain unchanged. For any US inquiries regarding orders or support during this transition, reach out to Cosmo Bio: https://www.cosmobiousa.com/. For Non-US inquiries reach out to MBL in Japan: https://www.mblbio.com/.
PD048
Antibody
100 µl
This antibody reacts with Oryza sativa Transport inhibitor response 1 (OsTIR1).
| Target | OsTIR1 |
|---|---|
| Product Type | Antibody |
| Application | IC, WB |
| Clonality | Polyclonal |
| Conjugate | Unlabeled |
| Isotype | IgG |
| Immunogen | Recombinant full-length Oryza sativa Transport inhibitor response 1 (OsTIR1) protein |
| Host Species | Rabbit |
| Species Reactivity | Oryza sativa |
| Formulation | PBS containing 50% Glycerol (pH 7.2). No preservative is contained. |
| Research Area | Cell Biology, Ubiquitin-Proteasome |
| Description/Background | The auxin-inducible degron (AID) system allows the rapid and reversible proteolysis of proteins of interest, which have been invented by Dr. Masato Kanemaki in National Institute of Genetics in Japan. OsTIR1 is a F-box family protein specifically expressed in a rice plant. Since OsTIR1 can function under a culture condition at 37 degree, it is integrated in the AID system to work it in mammalian cells. Briefly, OsTIR1 binds to endogenous Skp1 to form the Skp1-Cullin1-OsTIR1 complex (SCFTIR1). SCFTIR1 binds and ubiquitinates the AID tag in the presence of auxin, and promotes proteasome-dependent degradation of the AID-tagged target protein. Human HCT116 cell lines harboring OsTIR1 (CMV-OsTIR1 RCB4662 or Tet-OsTIR1) are available from the RIKEN Cell Bank. |
| Storage Temperature | This antibody solution is stable for one year from the date of purchase when stored at -20°C. |
| Protocols | IC, WB |
| Source | Purified IgG from rabbit serum |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
PD048
Antibody
100 µl