Images
Western Blot
Note

Application Data with Unconjugated Antibody

Western Blot

Western blot analysis of Abeta42 fibrils (Lane 1, Lane 3) and prefibrillar oligomers (Lane 2, Lane 4) with APP fibrils polyclonal antibody(Cat # PAB28910; Lane 1, Lane 2) and APP oligomers polyclonal antibody (Cat # PAB28928; Lane 3, Lane 4).

Dot Blot
Note

Application Data with Unconjugated Antibody

Dot Blot

Dot blot analysis of Abeta42 and polyQ36 prefibrillar oligomers and fibrils with APP oligomers polyclonal antibody (Cat # PAB28928) and APP fibrils polyclonal antibody (Cat # PAB28910).

  • Specifications

    Product Description

    Rabbit polyclonal antibody against amyloid oligomers.

    Immunogen

    Synthetic molecular mimic of soluble APP oligomers.

    Host

    Rabbit

    Reactivity

    Human, Mouse, Rat

    Specificity

    PAB28929 recognizes all types of amyloid oligomers, likely with a peptide backbone epitope that is common in amyloid oligomers but not in native proteins, amyloidogenic monomer or mature amyloid fibrils. A 1:1000 dilution of PAB28929 was sufficient for detection of amyloid oligomers in 10ug of mouse brain lysates by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

    Form

    Liquid

    Conjugation

    ATTO 390

    Purification

    Protein A affinity purification

    Recommend Usage

    Dot blot
    ELISA
    Immunofluorescence
    Immunohistochemistry (1:1000-10000)
    Immunoprecipitation (1:1000)
    Western Blot
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    PBS pH7.4, 50% glycerol and 0.09% sodium azide

    Storage Instruction

    Store at 4°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    Application Data with Unconjugated Antibody

    Western Blot

    Western blot analysis of Abeta42 fibrils (Lane 1, Lane 3) and prefibrillar oligomers (Lane 2, Lane 4) with APP fibrils polyclonal antibody(Cat # PAB28910; Lane 1, Lane 2) and APP oligomers polyclonal antibody (Cat # PAB28928; Lane 3, Lane 4).

    Dot Blot

    Dot blot analysis of Abeta42 and polyQ36 prefibrillar oligomers and fibrils with APP oligomers polyclonal antibody (Cat # PAB28928) and APP fibrils polyclonal antibody (Cat # PAB28910).

  • Applications

    Western Blot

    Immunohistochemistry

    Immunofluorescence

    Immunoprecipitation

    Protocol Download

    Enzyme-linked Immunoabsorbent Assay

    Dot Blot

  • Gene Info — APP

    Entrez GeneID

    351

    Gene Name

    APP

    Gene Alias

    AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN2

    Gene Description

    amyloid beta (A4) precursor protein

    Omim ID

    104760 605714

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq

    Other Designations

    A4 amyloid protein|amyloid beta A4 protein|amyloid-beta protein|beta-amyloid peptide|cerebral vascular amyloid peptide|peptidase nexin-II|protease nexin-II

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