Aqp4 polyclonal antibody (ATTO 488)

Product Description

Rabbit polyclonal antibody raised against recombinant Rat Aqp4.

Immunogen

Recombinant protein corresponding to Rat Aqp4 C-terminus.

Sequence

CTKGSYMEVEDNRSQVETED

Host

Rabbit

Reactivity

Human, Mouse, Rat

Specificity

0.5 ug/mL of PAB28894 was sufficient for detection of Aqp4, ~35kDa and sometimes larger glycosylated bands ~50kDa, in 10ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Form

Liquid

Conjugation

ATTO 488

Purification

Affinity purification

Recommend Usage

Immunofluorescence (1:400)
Western Blot (1:1000-4000)
The optimal working dilution should be determined by the end user.

Storage Buffer

PBS pH7.4, 50% glycerol and 0.09% sodium azide

Storage Instruction

Store at -20°C.
Aliquot to avoid repeated freezing and thawing.

Note

Application Data with Unconjugated Antibody

Immunofluorescence

Immunofluorescence staining of Aqp4 in rat kidney with Aqp4 polyclonal antibody (Cat # PAB28892) at 1:200 dilution, DAPI merged.