Pure Artocarpus integrifolia (Jacalin) is a lectin composed of four subunits, two of ~10,000D and two of ~16,000D each. This 50,000D glycoprotein appears to bind only O-glycosidically linked oligosaccharides, preferring the structure galactosyl (b-1,3) N-acetylgalactosamine. This structure (the so-called "T-antigen") is the oligosaccharide to which peanut agglutinin binds. Unlike PNA, Jacalin will bind this structure even in a mono- or disialylated form. Jacalin has been used to purify human IgA, since no other human immunoglobulin class binds to Jacalin. The specificity of Jacalin also affords the opportunity to localize or isolate glycoproteins with O-glycosidically linked oligosaccharide side chains.||Activity:|<0.5ug/ml will agglutinate neuraminidase treated human erythrocytes.||Carbohydrate Specificity:|alpha-Galactose||Inhibitory Carbohydrate:|alpha-Galactose||Applications:|Suitable for use in IgA-binding and purification. Other applications not tested.||Recommended Dilutions:|Jacalin tends to be less soluble with decreasing pH. Maximum solubility occurs between pH 8-9. Optimal dilutions to be determined by researcher.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 6 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. |For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.