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> beta actin Polyclonal Antibody

beta actin Polyclonal Antibody

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Katalog-Nummer E-AB-40517-100

Size : 100uL

Marke : Elabscience

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Verified Samples Verified Samples in WB: HT-29, Jurkat, Mouse spleen, Rat spleen, Mouse lung, Rat lung, RAW264.7, C6, Zebrafish, Monkey Vero
Verified Samples in IF: SH-SY5Y
Dilution WB 1:1000-2000,  IF 1:100-400
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse,  Rat,  Zebrafish,  Monkey
Applications WB,  IF
Clonality Polyclonal
Immunogen Recombinant human Beta actin protein.
Abbre beta actin
Synonyms A26C1A,   Actin beta,   Actin cytoplasmic 1,   Actx,   b actin,   β-Actin,  ACTB
Swissprot
Calculated MW 43 kDa
Observed MW 43 kDa

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization Cytoplasm, Cytoskeleton, Nucleus.
Concentration 2 mg/mL
Buffer PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method Antigen Affinity Purification
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background Beta-actin (ACTB/ACTB) is one of six different actin isoforms in humans. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actin is a major constituent of the contractile apparatus and one of th
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Other Clones

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HRP

Unconjugated

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