Immunohistochemistry of Human Tonsil using Anti-Lambda Light Chain antibody RM127. ELISA of human immunoglobulins shows RM127 reacts to the lambda light chain of human immunoglobulins. No cross reactivity with the kappa light chain, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of RM127 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. A titer ELISA using RM127. The plate was coated with different amounts of human IgG3λ. A serial dilution of RM127 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Product Details Product Name Biotin Anti-Human Lambda Light Chain Rabbit Monoclonal Antibody, Clone RM127 Description Biotinylated Rabbit monoclonal to Human Lambda Light Chain Catalog No. 31-1029-02 Clone Name RM127 Product Data Sheet Biotin Anti-Human Lambda Light Chain Rabbit Monoclonal Antibody Data Sheet RM127 Specificity RM127 reacts to the lambda light chain of human immunoglobulins. No cross reactivity with kappa light chain, mouse IgG, rat IgG, or goat IgG Immunogen Human IgG Application Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA Species Reactivity Human Conjugate Biotin Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Item Size 50 µg Concentration 1.0 mg/mL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage ELISA: 0.05 µg/mL – 0.2 µg/mL;
Immunocytochemistry (ICC): 0.5 µg/mL-2 µg/mL;
Immunohistochemistry (IHC): 0.5 µg/mL-2 µg/mL;
Flow Cytometry (FC): 0.2ug/mL-2ug/mL. Keywords . Product Images
Biotin Anti-Human Lambda Light Chain rabbit monoclonal antibody [RM127]
Katalog-Nummer 31-1029-02
Size : 50ug
Marke : RevMab Biosciences
Biotinylated Rabbit monoclonal to Human Ig lambda light chain
50 µg