Blood Ammonia Colorimetric Assay Kit
Katalog-Nummer E-BC-K145-S-50
Size : 50Assays
Marke : Elabscience
Contact local distributor :
Telefonnummer : +1 850 650 7790
Detection principle
Blood protein can be precipitated with protein precipitator, and enzyme activity will be destroyed, which can prevent the formation of free ammonia in vitro. Most interfering color substances were removed at the same time, indigo was formed in non-protein filtrate by Berthelot reaction, and the color depth was proportional to the content of blood ammonia. Blood ammonia content can be determined by comparing with standard solution.
Performance characteristics
| Synonyms | NH3 |
| Sample type | Serum,plasma |
| Sensitivity | 0.01 mmol/L |
| Detection range | 0.01-2.0 mmol/L |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 65 min |
| Precision | Average inter-assay CV: 5%Average intra-assay CV: 4.700% |
| Other instruments required | Micropipettor, Vortex mixer, Incubator, Centrifuge |
| Other reagents required | Normal saline (0.9% NaCl) |
| Storage | 2-8℃ |
| Valid period | 12 months |
Troubleshooting
Collection and treatment of common samples for metabolism assay detection
Questions and Answers of Metabolism Assay Kits
Citations
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NEUROSCIENCE LETTERS (2021) IF: 3.046
Syringic acid protects against thioacetamide-induced hepatic encephalopathy: Behavioral, biochemical, and molecular evidencePMID: 34871743
Sample: Serum -
METABOLIC BRAIN DISEASE (2022) IF: 3.584
Hepatoprotective and neuroprotective effect of taxifolin on hepatic encephalopathy in ratsPMID: 35298730
Sample: Serum -
Frontiers in Medicine (2020) IF: 3.9
Helicobacter pylori as an Initiating Factor of Complications in Patients With Cirrhosis: A Single-Center Observational StudySample: Serum